Whole-genome sequencing and characterization of an antibiotic resistant Neisseria meningitidis B isolate from a military unit in Vietnam

Tran, Thach Xuan, Le, Trang Thu, Trieu, Long Phi, Austin, Christopher M, Van Quyen, Dong and Nguyen, Huong Minh 2019, Whole-genome sequencing and characterization of an antibiotic resistant Neisseria meningitidis B isolate from a military unit in Vietnam, Annals of clinical microbiology and antimicrobials, vol. 18, pp. 1-9, doi: 10.1186/s12941-019-0315-z.

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Title Whole-genome sequencing and characterization of an antibiotic resistant Neisseria meningitidis B isolate from a military unit in Vietnam
Author(s) Tran, Thach Xuan
Le, Trang Thu
Trieu, Long Phi
Austin, Christopher MORCID iD for Austin, Christopher M orcid.org/0000-0003-1848-6267
Van Quyen, Dong
Nguyen, Huong Minh
Journal name Annals of clinical microbiology and antimicrobials
Volume number 18
Article ID 16
Start page 1
End page 9
Total pages 9
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2019
ISSN 1476-0711
1476-0711
Keyword(s) Antibiotic resistance
Antigen sequence typing
Epidemiological characterization
Neisseria meningitidis
Next generation sequencing
Vietnam
Science & Technology
Life Sciences & Biomedicine
Microbiology
Summary BACKGROUND: Invasive meningococcal disease (IMD) persists in military units in Vietnam despite the availability of antibiotics and vaccines. A hindrance to reducing the incidence of IMD in Vietnam is a lack of molecular data from isolates of the causative agent, Neisseria meningitidis from this country. Here, we characterized key genetic and epidemiological features of an invasive N. meningitidis isolate from a military unit in Vietnam using whole-genome sequencing. METHODS: Neisseria meningitidis was isolated from a conscript admitted for meningitis and tested against seven antibiotics. DNA from the isolate was extracted and sequenced using the Illumina HiSeq platform. Denovo assembly and scaffolding were performed to construct a draft genome assembly, from which genes were predicted and functionally annotated. Genome analysis included epidemiological characterization, genomic composition and identification of antibiotic resistance genes. RESULTS: Susceptibility testing of the isolate showed high levels of resistance to chloramphenicol and diminished susceptibility to ampicillin and rifampicin. A draft genome of ~ 2.1 Mb was assembled, containing 2451 protein coding sequences, 49 tRNAs and 3 rRNAs. Fifteen coding sequences sharing ≥ 84% identity with known antibiotic resistance genes were identified. Genome analysis revealed abundant repetitive DNAs and two prophages. Epidemiological typing revealed newly described sequence type, antigenic finetype and Bexsero® Antigen Sequence Typing (BAST). The BAST profile showed no coverage by either Bexsero® or Trumenba®. CONCLUSIONS: Our results present the first genome assembly of an invasive N. meningitidis isolate from a military unit in Vietnam. This study illustrates the usefulness of whole genome sequencing (WGS) analysis for epidemiological and antibiotic resistance studies and surveillance of IMD in Vietnam.
Language eng
DOI 10.1186/s12941-019-0315-z
Field of Research 1103 Clinical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Author(s)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30121493

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