The inner workings of the outer surface: skin and gill microbiota as indicators of changing gut health in Yellowtail Kingfish Legrand, Thibault P. R. A., Catalano, Sarah R., Wos-Oxley, Melissa L., Stephens, Fran, Landos, Matt, Bansemer, Matthew S., Stone, David A. J., Qin, Jian G. and Oxley, Andrew 2018, The inner workings of the outer surface: skin and gill microbiota as indicators of changing gut health in Yellowtail Kingfish, Frontiers in microbiology, vol. 8, doi: 10.3389/fmicb.2017.02664. Attached Files Name Description MIMEType Size Downloads Title The inner workings of the outer surface: skin and gill microbiota as indicators of changing gut health in Yellowtail Kingfish Author(s) Legrand, Thibault P. R. A. Catalano, Sarah R. Wos-Oxley, Melissa L. Stephens, Fran Landos, Matt Bansemer, Matthew S. Stone, David A. J. Qin, Jian G. Oxley, Andrew orcid.org/0000-0002-6056-7602 Journal name Frontiers in microbiology Volume number 8 Article ID 2664 Total pages 17 Publisher Frontiers Place of publication Lausanne, Switzerland Publication date 2018-01-15 ISSN 1664-302X Summary © 2018 Legrand, Catalano, Wos-Oxley, Stephens, Landos, Bansemer, Stone, Qin and Oxley. The mucosal surfaces and associated microbiota of fish are an important primary barrier and provide the first line of defense against potential pathogens. An understanding of the skin and gill microbial assemblages and the factors which drive their composition may provide useful insights into the broad dynamics of fish host-microbial relationships, and may reveal underlying changes in health status. This is particularly pertinent to cultivated systems whereby various stressors may led to conditions (like enteritis) which impinge on productivity. As an economically important species, we assessed whether the outer-surface bacterial communities reflect a change in gut health status of cultivated Yellowtail Kingfish (Seriola lalandi). Active bacterial assemblages were surveyed from RNA extracts from swabs of the skin and gills by constructing Illumina 16S rRNA gene amplicon libraries. Proteobacteria and Bacteroidetes were predominant in both the skin and gills, with enrichment of key ß-proteobacteria in the gills (Nitrosomonadales and Ferrovales). Fish exhibiting early stage chronic lymphocytic enteritis comprised markedly different global bacterial assemblages compared to those deemed healthy and exhibiting late stages of the disease. This corresponded to an overall loss of diversity and enrichment of Proteobacteria and Actinobacteria, particularly in the gills. In contrast, bacterial assemblages of fish with late stage enteritis were generally similar to those of healthy individuals, though with some distinct taxa. In conclusion, gut health status is an important factor which defines the skin and gill bacterial assemblages of fish and likely reflects changes in immune states and barrier systems during the early onset of conditions like enteritis. This study represents the first to investigate the microbiota of the outer mucosal surfaces of fish in response to underlying chronic gut enteritis, revealing potential biomarkers for assessing fish health in commercial aquaculture systems. Language eng DOI 10.3389/fmicb.2017.02664 Indigenous content off HERDC Research category C1.1 Refereed article in a scholarly journal ERA Research output type C Journal article Copyright notice ©2018, Legrand, Catalano, Wos-Oxley, Stephens, Landos, Bansemer, Stone, Qin and Oxley. Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30124161 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)   Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Fri, 28 Jun 2019, 14:57:54 EST Fri, 28 Jun 2019, 15:40:22 EST Fri, 28 Jun 2019, 15:49:58 EST Mon, 01 Jul 2019, 09:53:40 EST Sat, 06 Jul 2019, 02:31:31 EST Fri, 16 Aug 2019, 06:02:29 EST Thu, 22 Aug 2019, 10:06:59 EST Thu, 22 Aug 2019, 10:12:07 EST Fri, 15 Nov 2019, 14:37:53 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 8 times in Scopus Search Google Scholar Access Statistics: 35 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Fri, 28 Jun 2019, 14:57:54 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.