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Sex-specific expression of alternative transcripts in Drosophila

McIntyre, Lauren M., Bono, Lisa M., Genissel, Anne, Westerman, Rick, Junk, Damion, Telonis-Scott, Marina, Harshman, Larry, Wayne, Marta L., Kopp, Artyom and Nuzhdin, Sergey V. 2006, Sex-specific expression of alternative transcripts in Drosophila, Genome biology, vol. 7, no. 8, doi: 10.1186/gb-2006-7-8-R79.

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Title Sex-specific expression of alternative transcripts in Drosophila
Author(s) McIntyre, Lauren M.
Bono, Lisa M.
Genissel, Anne
Westerman, Rick
Junk, Damion
Telonis-Scott, MarinaORCID iD for Telonis-Scott, Marina orcid.org/0000-0002-8524-5538
Harshman, Larry
Wayne, Marta L.
Kopp, Artyom
Nuzhdin, Sergey V.
Journal name Genome biology
Volume number 7
Issue number 8
Article ID R79
Total pages 17
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2006-08-25
ISSN 1474-760X
1474-760X
Keyword(s) Alternative Splicing
Analysis of Variance
Animals
Drosophila melanogaster
Female
Gene Expression
Gene Expression Profiling
Genomics
Male
Oligonucleotide Array Sequence Analysis
Sex Characteristics
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Genetics & Heredity
QUANTITATIVE TRAIT LOCI
BIASED GENE-EXPRESSION
SPLICED MESSENGER-RNAS
WHOLE-GENOME ANALYSIS
FALSE DISCOVERY RATE
AXON GUIDANCE
CAENORHABDITIS-ELEGANS
MOLECULAR DIVERSITY
MELANOGASTER ADULTS
PROTEINS
Summary BACKGROUND: Many genes produce multiple transcripts due to alternative splicing or utilization of alternative transcription initiation/termination sites. This ’transcriptome expansion’ is thought to increase phenotypic complexity by allowing a single locus to produce several functionally distinct proteins. However, sex, genetic and developmental variation in the representation of alternative transcripts has never been examined systematically. Here, we describe a genome-wide analysis of sex-specific expression of alternative transcripts in Drosophila melanogaster. RESULTS: We compared transcript profiles in males and females from eight Drosophila lines (OregonR and 2b, and 6 RIL) using a newly designed 60-mer oligonucleotide microarray that allows us to distinguish a large proportion of alternative transcripts. The new microarray incorporates 7,207 oligonucleotides, satisfying stringent binding and specificity criteria that target both the common and the unique regions of 2,768 multi-transcript genes, as well as 12,912 oligonucleotides that target genes with a single known transcript. We estimate that up to 22% of genes that produce multiple transcripts show a sex-specific bias in the representation of alternative transcripts. Sexual dimorphism in overall transcript abundance was evident for 53% of genes. The X chromosome contains a significantly higher proportion of genes with female-biased transcription than the autosomes. However, genes on the X chromosome are no more likely to have a sexual bias in alternative transcript representation than autosomal genes. CONCLUSION: Widespread sex-specific expression of alternative transcripts in Drosophila suggests that a new level of sexual dimorphism at the molecular level exists.
Language eng
DOI 10.1186/gb-2006-7-8-R79
Indigenous content off
Field of Research 05 Environmental Sciences
06 Biological Sciences
08 Information and Computing Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Copyright notice ©2006, McIntyre et al.
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30126319

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.