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Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men

Vella, Luke, Markworth, James F., Farnfield, Michelle M., Maddipati, Krishna R., Russell, Aaron P. and Cameron-Smith, David 2019, Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men, Physiological Reports, vol. 7, no. 13, pp. 1-13, doi: 10.14814/phy2.14108.

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Title Intramuscular inflammatory and resolving lipid profile responses to an acute bout of resistance exercise in men
Author(s) Vella, Luke
Markworth, James F.
Farnfield, Michelle M.
Maddipati, Krishna R.
Russell, Aaron P.ORCID iD for Russell, Aaron P. orcid.org/0000-0002-7323-9501
Cameron-Smith, David
Journal name Physiological Reports
Volume number 7
Issue number 13
Article ID e14108
Start page 1
End page 13
Total pages 13
Publisher Wiley
Place of publication Hoboken, NJ
Publication date 2019-07
ISSN 2051-817X
Keyword(s) exercise recovery
inflammation
inflammation resolution
lipids
Science & Technology
Life Sciences & Biomedicine
Physiology
inflammatory resolution
SKELETAL-MUSCLE
ARACHIDONIC-ACID
AMNIOTIC-FLUID
LIPOXIN A(4)
RESOLUTION
IBUPROFEN
12-LIPOXYGENASE
TERM
OMEGA-3-FATTY-ACIDS
SUPPLEMENTATION
Summary Physiological Reports published by Wiley Periodicals, Inc. on behalf of The Physiological Society and the American Physiological Society. Lipid mediators including classical arachidonic acid-derived eicosanoids (e.g. prostaglandins and leukotrienes) and more recently identified specialized pro-resolving-mediator metabolites of the omega-3 fatty acids play essential roles in initiation, self-limitation, and active resolution of acute inflammatory responses. In this study, we examined the bioactive lipid mediator profile of human skeletal muscle at rest and following acute resistance exercise. Twelve male subjects completed a single bout of maximal isokinetic unilateral knee extension exercise and muscle biopsies were taken from the m.vastus lateralis before and at 2, 4, and 24 h of recovery. Muscle tissue lipid mediator profile was analyzed via liquid chromatography–mass spectrometry (LC-MS)-based targeted lipidomics. At 2 h postexercise, there was an increased intramuscular abundance of cyclooxygenase (COX)-derived thromboxanes (TXB2: 3.33 fold) and prostaglandins (PGE2: 2.52 fold and PGF2α: 1.77 fold). Resistance exercise also transiently increased muscle concentrations of lipoxygenase (LOX) pathway-derived leukotrienes (12-Oxo LTB4: 1.49 fold and 20-COOH LTB4: 2.91 fold), monohydroxy-eicosatetraenoic acids (5-HETE: 2.66 fold, 12-HETE: 2.83 fold, and 15-HETE: 1.69 fold) and monohydroxy-docosahexaenoic acids (4-HDoHE: 1.69 fold, 7-HDoHE: 1.58 fold and 14-HDoHE: 2.35 fold). Furthermore, the abundance of CYP pathway-derived epoxy- and dihydroxy-eicosatrienoic acids was increased in 2 h postexercise biopsies (5,6-EpETrE: 2.48 fold, 11,12-DiHETrE: 1.66 fold and 14,15-DiHETrE: 2.23 fold). These data reveal a range of bioactive lipid mediators as present within human skeletal muscle tissue and demonstrate that acute resistance exercise transiently stimulates the local production of both proinflammatory eicosanoids and pathway markers in specialized proresolving mediator biosynthesis circuits.
Language eng
DOI 10.14814/phy2.14108
Indigenous content off
Field of Research 0606 Physiology
1103 Clinical Sciences
1116 Medical Physiology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Authors
Free to Read? Yes
Use Rights 2019, The Author(s)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30128303

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.