A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains

Dixit, Pooja, Basu, Biswajit, Puri, Munish, Tuli, Deepak Kumar, Mathur, Anshu Shankar and Barrow, Colin James 2019, A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains, Biotechnology for biofuels, vol. 12, pp. 1-16, doi: 10.1186/s13068-019-1526-4.

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Title A screening approach for assessing lytic polysaccharide monooxygenase activity in fungal strains
Author(s) Dixit, Pooja
Basu, Biswajit
Puri, MunishORCID iD for Puri, Munish orcid.org/0000-0003-2469-3326
Tuli, Deepak Kumar
Mathur, Anshu Shankar
Barrow, Colin JamesORCID iD for Barrow, Colin James orcid.org/0000-0002-2153-7267
Journal name Biotechnology for biofuels
Volume number 12
Article ID 185
Start page 1
End page 16
Total pages 16
Publisher BioMed Central
Place of publication London, Eng.
Publication date 2019
ISSN 1754-6834
Keyword(s) AA9 LPMOs
Penicillium sp. LPMOs
Synergy in cellulases
Acid-treated rice straw
Gluconic acid
Cellobionic acid
Science & Technology
Life Sciences & Biomedicine
Biotechnology & Applied Microbiology
Energy & Fuels
Penicillium sp
Summary Background: Efforts to develop efficient lignocellulose-degrading enzymatic preparations have led to the relatively recent discovery of a new class of novel cellulase boosters, termed lytic polysaccharide monoxygenases (LPMOs). These enzymes are copper-dependent metalloenzymes that initiate the biomass deconstruction process and subsequently work together with cellulases, hemicellulases, and other accessory enzymes to enhance their hydrolytic action. Given their wide distribution and diversity, screening and isolation of potent LPMOs from natural fungal diversity may provide an important avenue for increasing the efficiency of cellulases and thereby decreasing cellulosic ethanol production costs. However, methods for quick screening and detection are still not widely available. In this article, a simple and sensitive method is described by combining nonhydrolytic activity enhancement followed by LC-MS-based quantitation of LPMOs. Results: In this study, a screening approach has been developed for the detection of nonhydrolytic cellulase-enhancing enzymes in crude fungal supernatants. With the application of a saturating benchmark cocktail of Celluclast 1.5L, fungal isolates were selected which had the capability of hydrolyzing pretreated rice straw by their synergistic enzyme fractions. Subsequently, these fungal extracts along with an LPMO-enriched commercial enzyme were investigated for their ability to produce Type I LPMO activity. An LC-MS-based methodology was applied to quantitate gluconic acid in enzymatic hydrolysates as an indirect measurement of Type I LPMO activity. Conclusion: The present study describes an LC-MS-based separation method to detect and quantitate LPMO activity in a commercial enzyme. This method was also applied to screen fungal extracts. The developed screening strategy has enabled detection of LPMO activity in two industrially important Penicillium strains.
Language eng
DOI 10.1186/s13068-019-1526-4
Indigenous content off
Field of Research 0904 Chemical Engineering
1003 Industrial Biotechnology
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Author(s)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30128554

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