Insight Into the microbial co-occurrence and diversity of 73 grapevine (Vitis vinifera) crown galls collected across the northern hemisphere

Gan, Han Ming, Szegedi, Erno, Fersi, Rabeb, Chebil, Samir, Kovacs, Laszlo, Kawaguchi, Akira, Hudson, Andre O., Burr, Thomas J. and Savka, Michael A. 2019, Insight Into the microbial co-occurrence and diversity of 73 grapevine (Vitis vinifera) crown galls collected across the northern hemisphere, Frontiers in microbiology, vol. 10, pp. 1-14, doi: 10.3389/fmicb.2019.01896.

Attached Files
Name Description MIMEType Size Downloads

Title Insight Into the microbial co-occurrence and diversity of 73 grapevine (Vitis vinifera) crown galls collected across the northern hemisphere
Formatted title Insight Into the microbial co-occurrence and diversity of 73 grapevine (Vitis vinifera) crown galls collected across the northern hemisphere 
Author(s) Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Szegedi, Erno
Fersi, Rabeb
Chebil, Samir
Kovacs, Laszlo
Kawaguchi, Akira
Hudson, Andre O.
Burr, Thomas J.
Savka, Michael A.
Journal name Frontiers in microbiology
Volume number 10
Article ID 1896
Start page 1
End page 14
Total pages 14
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2019-08
ISSN 1664-302X
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Microbiology
Agrobacterium
Allorhizobium vitis
amplicon sequence variants
crown gall disease
grape
microbiota
opine
vineyard
Summary Crown gall (CG) is a globally distributed and economically important disease of grapevine and other important crop plants. The causal agent of CG is Agrobacterium or Allorhizobium strains that harbor a tumor-inducing plasmid (pTi). The microbial community within the CG tumor has not been widely elucidated and it is not known if certain members of this microbial community promote or inhibit CG. This study investigated the microbiotas of grapevine CG tumor tissues from seven infected vineyards located in Hungary, Japan, Tunisia, and the United States. Heavy co-amplification of grapevine chloroplast and mitochondrial ribosomal RNA genes was observed with the widely used Illumina V3–V4 16S rRNA gene primers, requiring the design of a new reverse primer to enrich for bacterial 16S rRNA from CG tumors. The operational taxonomic unit (OTU) clustering approach is not suitable for CG microbiota analysis as it collapsed several ecologically distinct Agrobacterium species into a single OTU due to low interspecies genetic divergence. The CG microbial community assemblages were significantly different across sampling sites (ANOSIM global R = 0.63, p-value = 0.001) with evidence of site-specific differentially abundant ASVs. The presence of Allorhizobium vitis in the CG microbiota is almost always accompanied by Xanthomonas and Novosphingobium, the latter may promote the spread of pTi plasmid by way of acyl-homoserine lactone signal production, whereas the former may take advantage of the presence of substrates associated with plant cell wall growth and repair. The technical and biological insights gained from this study will contribute to the understanding of complex interaction between the grapevine and its microbial community and may facilitate better management of CG disease in the future.
Language eng
DOI 10.3389/fmicb.2019.01896
Indigenous content off
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2019, The Author(s)
Persistent URL http://hdl.handle.net/10536/DRO/DU:30129775

Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 23 Abstract Views, 2 File Downloads  -  Detailed Statistics
Created: Mon, 16 Sep 2019, 14:36:35 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.