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In vitro study on the effects of condensed tannins of different molecular weights on bovine rumen fungal population and diversity

Saminathan, Mookiah, Ramiah, Suriya Kumari, Gan, Han Ming, Abdullah, Norhani, Wong, Clement Michael Vui Ling, Ho, Yin Wan and Idrus, Zulkifli 2019, In vitro study on the effects of condensed tannins of different molecular weights on bovine rumen fungal population and diversity, Italian journal of animal science, vol. 18, no. 1, pp. 1451-1462, doi: 10.1080/1828051X.2019.1681304.

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Title In vitro study on the effects of condensed tannins of different molecular weights on bovine rumen fungal population and diversity
Author(s) Saminathan, Mookiah
Ramiah, Suriya Kumari
Gan, Han MingORCID iD for Gan, Han Ming orcid.org/0000-0001-7987-738X
Abdullah, Norhani
Wong, Clement Michael Vui Ling
Ho, Yin Wan
Idrus, Zulkifli
Journal name Italian journal of animal science
Volume number 18
Issue number 1
Start page 1451
End page 1462
Total pages 12
Publisher Taylor & Francis
Place of publication Abingdon, Eng.
Publication date 2019
ISSN 1594-4077
1828-051X
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Agriculture, Dairy & Animal Science
Agriculture, Multidisciplinary
Veterinary Sciences
Agriculture
Condensed tannin fractions
in vitro
rumen fungi
diversity
ITS-1 gene
LEUCAENA-LEUCOCEPHALA HYBRID
ANAEROBIC FUNGI
PHYLUM NEOCALLIMASTIGOMYCOTA
METHANE PRODUCTION
FRACTIONS
COMMUNITY
ITS1
ECOLOGY
WILD
Summary Condensed tannin (CT) of varying molecular weights (MWs) may affect rumen microbial fermentation by shifting composition of fungal community. In this study the effects of unfractionated CTs (F0) and CT fractions of different MWs (F1 > F2 > F3 > F4 > F5) from Leucaena leucocephala hybrid-Rendang (LLR) on the fungal mass and composition of fungal community were determined using molecular approaches. The results showed that the total fungi biomass decreased (p < .05) with the addition of higher-MWs CT fractions F1, F2 and F3 with values 35.3 µg/mL, 34.6 µg/mL and 39.3µg/mL, respectively, when compared to that of the control sample (without CTs) at 52.1 µg/mL. Sequencing of the polymorphic internal transcribed spacer 1 (ITS-1) by high-throughput sequencer, recovered mean of 109,190 sequences per-sample which comprised of a number of operation taxonomy units (OTUs) ranging from 22 to 38 and were classified into 7 genera. Piromyces 4 was the dominant genus in the control representing ∼70% of the sequences. Relative abundance of the Piromyces 4 increased significantly (p < .05) with increasing MWs of the CT fractions, however the second dominant Neocallimastigaceae was significantly (p < .05) reduced. This study demonstrated that CT fractions of different MWs could decrease the population mass as well as alter the rumen fungal community structure and diversity, and the effect was more pronounced for higher-MWs CTs. Hence, the shift in fungal diversity, accompanied by changes in population mass would influence fibre digestibility in the rumen in the presence of high MW CTs.
Language eng
DOI 10.1080/1828051X.2019.1681304
Indigenous content off
Field of Research 0702 Animal Production
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30132282

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.