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Akt1 is the principal Akt isoform regulating apoptosis in limiting cytokine concentrations

Green, B. D., Jabbour, A. M., Sandow, J. J., Riffkin, C. D., Masouras, D., Daunt, C. P., Salmanidis, M., Brumatti, G., Hemmings, B. A., Guthridge, M. A., Pearson, R. B. and Ekert, P. G. 2013, Akt1 is the principal Akt isoform regulating apoptosis in limiting cytokine concentrations, Cell death & differentiation, vol. 20, no. 10, pp. 1341-1349, doi: 10.1038/cdd.2013.63.

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Title Akt1 is the principal Akt isoform regulating apoptosis in limiting cytokine concentrations
Author(s) Green, B. D.
Jabbour, A. M.
Sandow, J. J.
Riffkin, C. D.
Masouras, D.
Daunt, C. P.
Salmanidis, M.
Brumatti, G.
Hemmings, B. A.
Guthridge, M. A.ORCID iD for Guthridge, M. A. orcid.org/0000-0002-0536-3471
Pearson, R. B.
Ekert, P. G.
Journal name Cell death & differentiation
Volume number 20
Issue number 10
Start page 1341
End page 1349
Total pages 9
Publisher Springer
Place of publication Cham, Switzerland
Publication date 2013
ISSN 1350-9047
1476-5403
Summary The activation of the Akt signalling in response to cytokine receptor signalling promotes protein synthesis, cellular growth and proliferation. To determine the role of Akt in interleukin-3 (IL-3) signalling, we generated IL-3-dependent myeloid cell lines from mice lacking Akt1, Akt2 or Akt3. Akt1 deletion resulted in accelerated apoptosis at low concentrations of IL-3. Expression of constitutively active Akt1 was sufficient to delay apoptosis in response to IL-3 withdrawal, but not sufficient to induce proliferation in the absence of IL-3. Akt1 prolonged survival of Bim- or Bad-deficient cells, but not cells lacking Puma, indicating that Akt1-dependent repression of apoptosis was in part dependent on Puma and independent of Bim or Bad. Our data show that a key role of Akt1 during IL-3 signalling is to repress p53-dependent apoptosis pathways, including transcriptional upregulation of Puma. Moreover, our data indicate that regulation of BH3-only proteins by Akt is dispensable for Akt-dependent cell survival.
Language eng
DOI 10.1038/cdd.2013.63
Indigenous content off
Field of Research 06 Biological Sciences
11 Medical and Health Sciences
HERDC Research category C1.1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30132349

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.