These shoes were made for walking (in blood): DNA transfer and the impact of different subfloor types

Taylor, Angela 2019, These shoes were made for walking (in blood): DNA transfer and the impact of different subfloor types, B.Forensic Science (Hons) thesis, School of Life and Environmental Sciences, Deakin University.

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Title These shoes were made for walking (in blood): DNA transfer and the impact of different subfloor types
Author Taylor, Angela
Institution Deakin University
School School of Life and Environmental Sciences
Faculty Faculty of Science, Engineering and Built Environment
Degree type Honours
Degree name B.Forensic Science (Hons)
Thesis advisor Durdle, AnnalisaORCID iD for Durdle, Annalisa
Date submitted 2019-11-08
Keyword(s) forensic
DNA transfer
DNA evidence
Summary Recently there has been research conducted into different aspects of DNA transfer, however, there is still a lack of understanding on certain aspects of this discipline. This research was designed to answer the question if human DNA from blood or latent blood could be detected past visualisation with the naked eye and visualisation with chemiluminescence, and if DNA could be detected, how much further could it be detected?
In this study, five participants created shoeprints from human blood over three different subfloor types (concrete, carpet, or linoleum). Observations of these shoeprints with the naked eye and with chemiluminescence using a luminol solution were recorded. Samples were taken from different shoeprints where chemiluminescence was either visible or not visible and then analysed for the presence of DNA, observing the quantity and quality of samples.
These results showed that there was the possibility of getting DNA past the point at which blood could no longer be visualised at a quantity high enough to generate a DNA profile. This research also found that the type of subfloor effected the quality and quantity of DNA obtained, and that there was no effect observed between participants. This demonstrates to forensic investigators the fact that although blood may not be visible with common visualisation methods, it may still be possible to obtain forensically useful DNA from crime scenes.
Language eng
Indigenous content off
Field of Research 0699 Other Biological Sciences
Description of original 65 p.
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