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FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression

Guthridge, Mark A, Bellosta, Paola, Tavoloni, Nicola and Basilico, Claudio 1997, FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression, MOLECULAR AND CELLULAR BIOLOGY, vol. 17, no. 9, pp. 5485-5498, doi: 10.1128/MCB.17.9.5485.

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Title FIN13, a novel growth factor-inducible serine-threonine phosphatase which can inhibit cell cycle progression
Author(s) Guthridge, Mark AORCID iD for Guthridge, Mark A orcid.org/0000-0002-0536-3471
Bellosta, Paola
Tavoloni, Nicola
Basilico, Claudio
Journal name MOLECULAR AND CELLULAR BIOLOGY
Volume number 17
Issue number 9
Start page 5485
End page 5498
Total pages 14
Publisher AMER SOC MICROBIOLOGY
Place of publication United States
Publication date 1997-09-01
ISSN 0270-7306
1098-5549
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Biochemistry & Molecular Biology
Cell Biology
PROTEIN PHOSPHATASES
MOLECULAR-CLONING
EXPRESSION
PHOSPHORYLATION
KINASES
SPECIFICITY
SEQUENCES
COLLAGEN
ENTRY
BETA
Summary We have identified a novel type 2C serine-threonine phosphatase, FIN13, whose expression is induced by fibroblast growth factor 4 and serum in late G1 phase. The protein encoded by FIN13 cDNA includes N- and C-terminal domains with significant homologies to type 2C phosphatases, a domain homologous to collagen, and an acidic domain. FIN13 expression predominates in proliferating tissues. Bacterially expressed FIN13 and FIN13 expressed in mammalian cells exhibit serine-threonine phosphatase activity, which requires Mn2+ and is insensitive to inhibition by okadaic acid. FIN13 is localized in the nuclei of transiently transfected cells. Cotransfection of FIN13-expressing plasmids with a plasmid that expresses the neomycin resistance gene inhibits the growth of drug-resistant colonies in NIH 3T3, HeLa and Rat-1 cells. In transiently transfected cells, FIN13 inhibits DNA synthesis and results in the accumulation of cells in G1 and early S phases. Similarly, the induction of expression of FIN13 under the control of a tetracycline-regulated promoter in NIH 3T3 cells leads to growth inhibition, with accumulation of cells in G1 and early S phases. Thus, overexpression and/or unregulated expression of FIN13 inhibits cell cycle progression, indicating that the physiological role of this phosphatase may be that of regulating the orderly progression of cells through the mitotic cycle by dephosphorylating specific substrates which are important for cell proliferation.
Language eng
DOI 10.1128/MCB.17.9.5485
Indigenous content off
Field of Research 06 Biological Sciences
11 Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30134396

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.