Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity Mosaheb, MM, Dobrikova, EY, Brown, MC, Yang, Y, Cable, J, Okada, H, Nair, SK, Bigner, DD, Ashley, DM and Gromeier, M 2020, Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity, Nature Communications, vol. 11, no. 1, pp. 1-15, doi: 10.1038/s41467-019-13939-z. Attached Files Name Description MIMEType Size Downloads ashley-geneticallystablepoliovirus-2020.pdf Published version application/pdf 4.93MB 10 Title Genetically stable poliovirus vectors activate dendritic cells and prime antitumor CD8 T cell immunity Author(s) Mosaheb, MM Dobrikova, EY Brown, MC Yang, Y Cable, J Okada, H Nair, SK Bigner, DD Ashley, DM Gromeier, M Journal name Nature Communications Volume number 11 Issue number 1 Article ID 524 Start page 1 End page 15 Total pages 15 Publisher Nature Research Place of publication Berlin, Germany Publication date 2020-01-27 ISSN 2041-1723 Keyword(s) Science & Technology Multidisciplinary Sciences Science & Technology - Other Topics TRANSLATION INITIATION EXPRESSION VECTORS I INTERFERON RNA VACCINE BINDING TOLERANCE SITE NEUROVIRULENCE PROLIFERATION Summary Viruses naturally engage innate immunity, induce antigen presentation, and mediate CD8 T cell priming against foreign antigens. Polioviruses can provide a context optimal for generating antigen-specific CD8 T cells, as they have natural tropism for dendritic cells, preeminent inducers of CD8 T cell immunity; elicit Th1-promoting inflammation; and lack interference with innate or adaptive immunity. However, notorious genetic instability and underlying neuropathogenicity has hampered poliovirus-based vector applications. Here we devised a strategy based on the polio:rhinovirus chimera PVSRIPO, devoid of viral neuropathogenicity after intracerebral inoculation in human subjects, for stable expression of exogenous antigens. PVSRIPO vectors infect, activate, and induce epitope presentation in DCs in vitro; they recruit and activate DCs with Th1-dominant cytokine profiles at the injection site in vivo. They efficiently prime tumor antigen-specific CD8 T cells in vivo, induce CD8 T cell migration to the tumor site, delay tumor growth and enhance survival in murine tumor models. Language eng DOI 10.1038/s41467-019-13939-z Indigenous content off HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2020, The Author(s) Free to Read? Yes Use Rights Persistent URL http://hdl.handle.net/10536/DRO/DU:30134756 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Fri, 07 Feb 2020, 12:37:09 EST Sat, 08 Feb 2020, 04:01:33 EST Mon, 10 Feb 2020, 07:10:36 EST Sat, 14 Mar 2020, 01:30:19 EST Tue, 12 Jan 2021, 05:02:13 EST Mon, 08 Feb 2021, 14:30:51 EST Mon, 08 Feb 2021, 14:32:48 EST Filtered Full Citation counts:   Cited 3 times in TR Web of Science Cited 2 times in Scopus Search Google Scholar Access Statistics: 14 Abstract Views, 10 File Downloads  -  Detailed Statistics Created: Fri, 07 Feb 2020, 12:37:09 EST

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