Effect of nonsteroidal anti-inflammatory drugs on LFA-1 and ICAM-1 expression in gastric mucosa

Andrews, F. J., Malcontenti-Wilson, C. and O'Brien, P. E. 1994, Effect of nonsteroidal anti-inflammatory drugs on LFA-1 and ICAM-1 expression in gastric mucosa, American Journal of Physiology - Gastrointestinal and Liver Physiology, vol. 266, no. 4, pp. G657-G664, doi: 10.1152/ajpgi.1994.266.4.g657.

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Title Effect of nonsteroidal anti-inflammatory drugs on LFA-1 and ICAM-1 expression in gastric mucosa
Author(s) Andrews, F. J.ORCID iD for Andrews, F. J. orcid.org/0000-0001-5082-1702
Malcontenti-Wilson, C.
O'Brien, P. E.
Journal name American Journal of Physiology - Gastrointestinal and Liver Physiology
Volume number 266
Issue number 4
Start page G657
End page G664
Total pages 8
Publisher American Physiological Society
Place of publication Rockville, Md.
Publication date 1994-01-01
ISSN 0002-9513
Summary Leukocyte adhesion to the endothelium appears to play an important role in gastric injury. This study aimed to develop immunohistochemical staining techniques to investigate the distribution and sequence of expression of both leukocyte [lymphocyte function associated antigen 1 (LFA-1)] and endothelial [intracellular adhesion molecule 1 (ICAM-1)] adhesion molecules in the mucosa after treatment with nonsteroidal anti-inflammatory drugs (NSAIDs). In control rats there were 803 ± 72 LFA-1-stained cells/mm2 in the deep mucosa, 134 ± 32 cells/mm2 in the superficial mucosa, and 6.4 ± 1.2 ICAM- 1-stained blood vessels/mm2 in the total mucosa. The number of ICAM-1- stained blood vessels in the mucosa increased significantly after 30 min of treatment with intragastric aspirin (30 mM; 25.2 ± 7.2/mm2, P < 0.01) and indomethacin (20 mg/kg; 20.7 ± 4.4/mm2, P < 0.01) before any appreciable mucosal damage was evident. This increase was reversed by treatment with misoprostol (100 μg/kg) in both aspirin- (7.6 ± 1.7/mm2, P < 0.01) and indomethacin-treated animals (10.7 ± 2.6/mm2, P < 0.05). There was no significant increase in LFA-1-positive cells until 60 min of NSAID treatment. We conclude that the adhesion molecules LFA-1 and ICAM-1 are expressed in the normal gastric mucosa and that the number of ICAM-1-stained blood vessels increases rapidly after NSAID treatment. This increase in ICAM-1 expression may be associated with an inhibition of prostaglandin synthesis by NSAIDs. These results provide further support for the role of early vascular changes in NSAID gastropathy.
DOI 10.1152/ajpgi.1994.266.4.g657
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HERDC Research category CN Other journal article
Copyright notice ©1994, American Physiological Society
Persistent URL http://hdl.handle.net/10536/DRO/DU:30134769

Document type: Journal Article
Collections: Faculty of Health
School of Health and Social Development
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