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The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding

Ch’ng, Sarah S., Walker, Adam J., McCarthy, Madeleine, Le, Thien-Kim, Thomas, Natalie, Gibbons, Andrew, Udawela, Madhara, Kusljic, Snezana, Dean, Brian and Gogos, Andrea 2020, The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding, Brain Sciences, vol. 10, no. 2, doi: 10.3390/brainsci10020106.

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Title The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding
Author(s) Ch’ng, Sarah S.
Walker, Adam J.ORCID iD for Walker, Adam J. orcid.org/0000-0002-5399-1245
McCarthy, Madeleine
Le, Thien-Kim
Thomas, Natalie
Gibbons, Andrew
Udawela, Madhara
Kusljic, Snezana
Dean, Brian
Gogos, Andrea
Journal name Brain Sciences
Volume number 10
Issue number 2
Article ID 106
Total pages 12
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2020-02-13
ISSN 2076-3425
2076-3425
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
ovariectomy
female
rat
schizophrenia
PPI
CHRM1
ACETYLCHOLINE-RECEPTORS
CAUDATE-PUTAMEN
PREFRONTAL CORTEX
ACOUSTIC STARTLE
NO CHANGE
ESTROGEN
AFFINITY
PROGESTERONE
EXPRESSION
Summary Ovarian hormones, such as estrogens and progesterone, are known to exert beneficial effects on cognition and some psychiatric disorders. The basis of these effects is not fully understood, but may involve altered cholinergic neurotransmission. This study aimed to investigate how a lack of ovarian hormones would impact muscarinic receptor-induced deficits in prepulse inhibition (PPI) and muscarinic receptor density in several brain regions. Adult female rats were either ovariectomized, to remove the source of ovarian hormones, or left intact (sham-operated). PPI is a measure of sensorimotor gating that is typically impaired in schizophrenia patients, and similar deficits can be induced in rats by administering scopolamine, a muscarinic receptor antagonist. Our results revealed no significant effects of ovariectomy on PPI after saline or scopolamine treatment. Autoradiography was performed to measure cholinergic muscarinic receptor binding density using [3H]-pirenzepine, [3H]-AF-DX, and [3H]-4-DAMP, to label M1, M2/M4, and M3 receptors, respectively. We examined the amygdala, caudate putamen, dorsal hippocampus, motor cortex, retrosplenial cortex, and ventromedial hypothalamus. There were no significant group differences in any region for any muscarinic receptor type. These results suggest that removing peripheral ovarian hormones does not influence the cholinergic muscarinic receptor system in the context of PPI or receptor binding density.
Language eng
DOI 10.3390/brainsci10020106
Indigenous content off
Field of Research 1109 Neurosciences
1701 Psychology
1702 Cognitive Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2020, The Authors
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30135513

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.