The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding Ch’ng, Sarah S., Walker, Adam J., McCarthy, Madeleine, Le, Thien-Kim, Thomas, Natalie, Gibbons, Andrew, Udawela, Madhara, Kusljic, Snezana, Dean, Brian and Gogos, Andrea 2020, The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding, Brain Sciences, vol. 10, no. 2, doi: 10.3390/brainsci10020106. Attached Files Name Description MIMEType Size Downloads Title The impact of removal of ovarian hormones on cholinergic muscarinic receptors: Examining prepulse inhibition and receptor binding Author(s) Ch’ng, Sarah S. Walker, Adam J. orcid.org/0000-0002-5399-1245 McCarthy, Madeleine Le, Thien-Kim Thomas, Natalie Gibbons, Andrew Udawela, Madhara Kusljic, Snezana Dean, Brian Gogos, Andrea Journal name Brain Sciences Volume number 10 Issue number 2 Article ID 106 Total pages 12 Publisher MDPI Place of publication Basel, Switzerland Publication date 2020-02-13 ISSN 2076-3425 2076-3425 Keyword(s) Science & Technology Life Sciences & Biomedicine Neurosciences Neurosciences & Neurology ovariectomy female rat schizophrenia PPI CHRM1 ACETYLCHOLINE-RECEPTORS CAUDATE-PUTAMEN PREFRONTAL CORTEX ACOUSTIC STARTLE NO CHANGE ESTROGEN AFFINITY PROGESTERONE EXPRESSION Summary Ovarian hormones, such as estrogens and progesterone, are known to exert beneficial effects on cognition and some psychiatric disorders. The basis of these effects is not fully understood, but may involve altered cholinergic neurotransmission. This study aimed to investigate how a lack of ovarian hormones would impact muscarinic receptor-induced deficits in prepulse inhibition (PPI) and muscarinic receptor density in several brain regions. Adult female rats were either ovariectomized, to remove the source of ovarian hormones, or left intact (sham-operated). PPI is a measure of sensorimotor gating that is typically impaired in schizophrenia patients, and similar deficits can be induced in rats by administering scopolamine, a muscarinic receptor antagonist. Our results revealed no significant effects of ovariectomy on PPI after saline or scopolamine treatment. Autoradiography was performed to measure cholinergic muscarinic receptor binding density using [3H]-pirenzepine, [3H]-AF-DX, and [3H]-4-DAMP, to label M1, M2/M4, and M3 receptors, respectively. We examined the amygdala, caudate putamen, dorsal hippocampus, motor cortex, retrosplenial cortex, and ventromedial hypothalamus. There were no significant group differences in any region for any muscarinic receptor type. These results suggest that removing peripheral ovarian hormones does not influence the cholinergic muscarinic receptor system in the context of PPI or receptor binding density. Language eng DOI 10.3390/brainsci10020106 Indigenous content off Field of Research 1109 Neurosciences 1701 Psychology 1702 Cognitive Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2020, The Authors Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30135513 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 09 Mar 2020, 09:27:34 EST Mon, 09 Mar 2020, 09:30:29 EST Mon, 09 Mar 2020, 09:31:37 EST Tue, 10 Mar 2020, 04:00:10 EST Tue, 10 Mar 2020, 07:01:00 EST Sat, 11 Apr 2020, 02:30:19 EST Mon, 07 Dec 2020, 05:02:04 EST Tue, 08 Dec 2020, 07:56:25 EST Tue, 08 Dec 2020, 08:00:04 EST Tue, 08 Dec 2020, 14:52:19 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 30 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Mon, 09 Mar 2020, 09:31:37 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.