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Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics

Yang, D, Zhang, W, Zhang, H, Zhang, F, Chen, L, Ma, L, Larcher, LM, Chen, S, Liu, N, Zhao, Q, Tran, HLP, Chen, C, Veedu, RN and Wang, T 2020, Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics, Theranostics, vol. 10, no. 8, pp. 3684-3707, doi: 10.7150/thno.41580.

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Title Progress, opportunity, and perspective on exosome isolation - efforts for efficient exosome-based theranostics
Author(s) Yang, D
Zhang, W
Zhang, H
Zhang, F
Chen, L
Ma, L
Larcher, LM
Chen, S
Liu, N
Zhao, Q
Tran, HLPORCID iD for Tran, HLP orcid.org/0000-0001-8463-7516
Chen, C
Veedu, RN
Wang, T
Journal name Theranostics
Volume number 10
Issue number 8
Start page 3684
End page 3707
Total pages 24
Publisher Ivyspring International Publisher
Place of publication Sydney, NSW
Publication date 2020-02-19
ISSN 1838-7640
1838-7640
Keyword(s) Exosome
diagnosis
extracellular vesicle
microfluidic
microvesicle
separation
Summary Exosomes are small extracellular vesicles with diameters of 30-150 nm. In both physiological and pathological conditions, nearly all types of cells can release exosomes, which play important roles in cell communication and epigenetic regulation by transporting crucial protein and genetic materials such as miRNA, mRNA, and DNA. Consequently, exosome-based disease diagnosis and therapeutic methods have been intensively investigated. However, as in any natural science field, the in-depth investigation of exosomes relies heavily on technological advances. Historically, the two main technical hindrances that have restricted the basic and applied researches of exosomes include, first, how to simplify the extraction and improve the yield of exosomes and, second, how to effectively distinguish exosomes from other extracellular vesicles, especially functional microvesicles. Over the past few decades, although a standardized exosome isolation method has still not become available, a number of techniques have been established through exploration of the biochemical and physicochemical features of exosomes. In this work, by comprehensively analyzing the progresses in exosome separation strategies, we provide a panoramic view of current exosome isolation techniques, providing perspectives toward the development of novel approaches for high-efficient exosome isolation from various types of biological matrices. In addition, from the perspective of exosome-based diagnosis and therapeutics, we emphasize the issue of quantitative exosome and microvesicle separation.
Language eng
DOI 10.7150/thno.41580
Indigenous content off
Field of Research 1112 Oncology and Carcinogenesis
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2020, the authors
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30136093

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.