Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection Vedururu, Ravi Kiran, Neave, Matthew J., Sundaramoorthy, Vinod, Green, Diane, Harper, Jennifer A., Gorry, Paul R., Duchemin, Jean-Bernard and Paradkar, Prasad N. 2019, Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection, Pathogens, vol. 8, no. 3, Special Issue: Chikungunya Virus Infections, pp. 1-12, doi: 10.3390/pathogens8030132. Attached Files Name Description MIMEType Size Downloads Title Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection Author(s) Vedururu, Ravi Kiran Neave, Matthew J. Sundaramoorthy, Vinod orcid.org/0000-0001-6309-8031 Green, Diane Harper, Jennifer A. Gorry, Paul R. Duchemin, Jean-Bernard Paradkar, Prasad N. Journal name Pathogens Volume number 8 Issue number 3 Season Special Issue: Chikungunya Virus Infections Article ID 132 Start page 1 End page 12 Total pages 12 Publisher MDPI Place of publication Basel, Switzerland Publication date 2019-09-01 ISSN 2076-0817 Keyword(s) Science & Technology Life Sciences & Biomedicine Microbiology Chikungunya Aedes albopictus RNASeq host-pathogen interactions Bruton's tyrosine kinase BTKi TYROSINE KINASE BTK Bruton’s tyrosine kinase host–pathogen interactions Summary Chikungunya virus (CHIKV) is transmitted by Aedes mosquitoes and causes prolonged arthralgia in patients. After crossing the mosquito midgut barrier, the virus disseminates to tissues including the head and salivary glands. To better understand the interaction between Aedes albopictus and CHIKV, we performed RNASeq analysis on pools of mosquito heads and parts of the thorax 8 days post infection, which identified 159 differentially expressed transcripts in infected mosquitos compared to uninfected controls. After validation using RT-qPCR (reverse transcriptase-quantitative polymerase chain reaction), inhibitor of Bruton’s tyrosine kinase (BTKi), which has previously been shown to be anti-inflammatory in mammals after viral infection, was further evaluated for its functional significance. Knockdown of BTKi using double-stranded RNA in a mosquito cell line showed no significant difference in viral RNA or infectivity titer. However, BTKi gene knocked-down cells showed increased apoptosis 24 hours post-infection compared with control cells, suggesting involvement of BTKi in the mosquito response to viral infection. Since BTK in mammals promotes an inflammatory response and has been shown to be involved in osteoclastogenesis, a hallmark of CHIKV pathogenesis, our results suggest a possible conserved mechanism at play between mosquitoes and mammals. Taken together, these results will add to our understanding of Aedes Albopictus interactions with CHIKV. Language eng DOI 10.3390/pathogens8030132 Indigenous content off Field of Research 110799 Immunology not elsewhere classified HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30136721 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 11 May 2020, 13:03:55 EST Tue, 12 May 2020, 05:03:11 EST Tue, 12 May 2020, 06:04:03 EST Mon, 18 May 2020, 11:51:05 EST Mon, 18 May 2020, 11:59:12 EST Mon, 18 May 2020, 11:59:50 EST Filtered Full Citation counts:   Cited 1 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 47 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 11 May 2020, 13:03:55 EST

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