Openly accessible

Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection

Vedururu, Ravi Kiran, Neave, Matthew J., Sundaramoorthy, Vinod, Green, Diane, Harper, Jennifer A., Gorry, Paul R., Duchemin, Jean-Bernard and Paradkar, Prasad N. 2019, Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection, Pathogens, vol. 8, no. 3, Special Issue: Chikungunya Virus Infections, pp. 1-12, doi: 10.3390/pathogens8030132.

Attached Files
Name Description MIMEType Size Downloads

Title Whole transcriptome analysis of Aedes albopictus mosquito head and thorax post-chikungunya virus infection
Author(s) Vedururu, Ravi Kiran
Neave, Matthew J.
Sundaramoorthy, VinodORCID iD for Sundaramoorthy, Vinod orcid.org/0000-0001-6309-8031
Green, Diane
Harper, Jennifer A.
Gorry, Paul R.
Duchemin, Jean-Bernard
Paradkar, Prasad N.
Journal name Pathogens
Volume number 8
Issue number 3
Season Special Issue: Chikungunya Virus Infections
Article ID 132
Start page 1
End page 12
Total pages 12
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2019-09-01
ISSN 2076-0817
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Microbiology
Chikungunya
Aedes albopictus
RNASeq
host-pathogen interactions
Bruton's tyrosine kinase
BTKi
TYROSINE KINASE
BTK
Bruton’s tyrosine kinase
host–pathogen interactions
Summary Chikungunya virus (CHIKV) is transmitted by Aedes mosquitoes and causes prolonged arthralgia in patients. After crossing the mosquito midgut barrier, the virus disseminates to tissues including the head and salivary glands. To better understand the interaction between Aedes albopictus and CHIKV, we performed RNASeq analysis on pools of mosquito heads and parts of the thorax 8 days post infection, which identified 159 differentially expressed transcripts in infected mosquitos compared to uninfected controls. After validation using RT-qPCR (reverse transcriptase-quantitative polymerase chain reaction), inhibitor of Bruton’s tyrosine kinase (BTKi), which has previously been shown to be anti-inflammatory in mammals after viral infection, was further evaluated for its functional significance. Knockdown of BTKi using double-stranded RNA in a mosquito cell line showed no significant difference in viral RNA or infectivity titer. However, BTKi gene knocked-down cells showed increased apoptosis 24 hours post-infection compared with control cells, suggesting involvement of BTKi in the mosquito response to viral infection. Since BTK in mammals promotes an inflammatory response and has been shown to be involved in osteoclastogenesis, a hallmark of CHIKV pathogenesis, our results suggest a possible conserved mechanism at play between mosquitoes and mammals. Taken together, these results will add to our understanding of Aedes Albopictus interactions with CHIKV.
Language eng
DOI 10.3390/pathogens8030132
Indigenous content off
Field of Research 110799 Immunology not elsewhere classified
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30136721

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
Connect to link resolver
 
Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved.

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.

Versions
Version Filter Type
Citation counts: TR Web of Science Citation Count  Cited 0 times in TR Web of Science
Scopus Citation Count Cited 0 times in Scopus
Google Scholar Search Google Scholar
Access Statistics: 14 Abstract Views, 0 File Downloads  -  Detailed Statistics
Created: Mon, 11 May 2020, 13:03:55 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.