Modelling Lyssavirus Infections in Human Stem Cell-Derived Neural Cultures Sundaramoorthy, Vinod, Godde, Nathan, Farr, Ryan J., Green, Diane, Haynes, John M., Bingham, John, O'Brien, Carmel M. and Dearnley, Megan 2020, Modelling Lyssavirus Infections in Human Stem Cell-Derived Neural Cultures, Viruses, vol. 12, no. 4, pp. 1-24, doi: 10.3390/v12040359. Attached Files Name Description MIMEType Size Downloads Title Modelling Lyssavirus Infections in Human Stem Cell-Derived Neural Cultures Author(s) Sundaramoorthy, Vinod orcid.org/0000-0001-6309-8031 Godde, Nathan Farr, Ryan J. Green, Diane Haynes, John M. Bingham, John O'Brien, Carmel M. Dearnley, Megan Journal name Viruses Volume number 12 Issue number 4 Start page 1 End page 24 Total pages 24 Publisher MDPI Place of publication Basel, Switzerland Publication date 2020-04-01 ISSN 1999-4915 1999-4915 Keyword(s) Science & Technology Life Sciences & Biomedicine Virology rabies lyssavirus stem cell-derived neurons ex-vivo models viral pathogenesis trans-synaptic axonal trafficking chemokine and cytokine response neuronal apoptosis BLOOD-BRAIN-BARRIER RABIES VIRUS PHOSPHOPROTEIN CENTRAL-NERVOUS-SYSTEM NEURONAL CELLS DIFFERENTIATION EXPRESSION PROTEIN ACTIVATION APOPTOSIS CCL5 Summary Rabies is a zoonotic neurological infection caused by lyssavirus that continues to result in devastating loss of human life. Many aspects of rabies pathogenesis in human neurons are not well understood. Lack of appropriate ex-vivo models for studying rabies infection in human neurons has contributed to this knowledge gap. In this study, we utilize advances in stem cell technology to characterize rabies infection in human stem cell-derived neurons. We show key cellular features of rabies infection in our human neural cultures, including upregulation of inflammatory chemokines, lack of neuronal apoptosis, and axonal transmission of viruses in neuronal networks. In addition, we highlight specific differences in cellular pathogenesis between laboratory-adapted and field strain lyssavirus. This study therefore defines the first stem cell-derived ex-vivo model system to study rabies pathogenesis in human neurons. This new model system demonstrates the potential for enabling an increased understanding of molecular mechanisms in human rabies, which could lead to improved control methods. Language eng DOI 10.3390/v12040359 Indigenous content off Field of Research 0605 Microbiology HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30136876 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 11 May 2020, 14:04:40 EST Tue, 12 May 2020, 05:05:57 EST Tue, 12 May 2020, 08:11:59 EST Sat, 04 Jul 2020, 02:30:42 EST Tue, 03 Nov 2020, 05:02:40 EST Tue, 03 Nov 2020, 11:02:12 EST Tue, 03 Nov 2020, 11:05:45 EST Tue, 03 Nov 2020, 11:06:46 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 32 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Mon, 11 May 2020, 14:04:40 EST

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