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Wet-Spun Trojan Horse Cell Constructs for Engineering Muscle

Quigley, Anita F., Cornock, Rhys, Mysore, Tharun, Foroughi, Javad, Kita, Magdalena, Razal, Joselito M., Crook, Jeremy, Moulton, Simon E., Wallace, Gordon G. and Kapsa, Robert M. I. 2020, Wet-Spun Trojan Horse Cell Constructs for Engineering Muscle, Frontiers in Chemistry, vol. 8, pp. 1-11, doi: 10.3389/fchem.2020.00018.

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Title Wet-Spun Trojan Horse Cell Constructs for Engineering Muscle
Author(s) Quigley, Anita F.
Cornock, Rhys
Mysore, Tharun
Foroughi, Javad
Kita, Magdalena
Razal, Joselito M.ORCID iD for Razal, Joselito M. orcid.org/0000-0002-9758-3702
Crook, Jeremy
Moulton, Simon E.
Wallace, Gordon G.
Kapsa, Robert M. I.
Journal name Frontiers in Chemistry
Volume number 8
Article ID 18
Start page 1
End page 11
Total pages 11
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2020-02-20
ISSN 2296-2646
Keyword(s) alginate fibers
myoblasts
wet-spun
muscle engineering
biosynthetic muscle scaffold
Science & Technology
Physical Sciences
Chemistry, Multidisciplinary
Chemistry
MUSCULAR-DYSTROPHY PATIENTS
IN-VIVO
FIBERS
EXPRESSION
INJECTIONS
Summary Engineering of 3D regenerative skeletal muscle tissue constructs (skMTCs) using hydrogels containing muscle precursor cells (MPCs) is of potential benefit for repairing Volumetric Muscle Loss (VML) arising from trauma (e.g., road/industrial accident, war injury) or for restoration of functional muscle mass in disease (e.g., Muscular Dystrophy, muscle atrophy). Additive Biofabrication (AdBiofab) technologies make possible fabrication of 3D regenerative skMTCs that can be tailored to specific delivery requirements of VML or functional muscle restoration. Whilst 3D printing is useful for printing constructs of many tissue types, the necessity of a balanced compromise between cell type, required construct size and material/fabrication process cyto-compatibility can make the choice of 3D printing a secondary alternative to other biofabrication methods such as wet-spinning. Alternatively, wet-spinning is more amenable to formation of fibers rather than (small) layered 3D-Printed constructs. This study describes the fabrication of biosynthetic alginate fibers containing MPCs and their use for delivery of dystrophin-expressing cells to dystrophic muscle in the mdx mouse model of Duchenne Muscular Dystrophy (DMD) compared to poly(DL-lactic-co-glycolic acid) copolymer (PLA:PLGA) topically-seeded with myoblasts. In addition, this study introduces a novel method by which to create 3D layered wet-spun alginate skMTCs for bulk mass delivery of MPCs to VML lesions. As such, this work introduces the concept of “Trojan Horse” Fiber MTCs (TH-fMTCs) and 3d Mesh-MTCs (TH-mMTCs) for delivery of regenerative MPCs to diseased and damaged muscle, respectively.
Language eng
DOI 10.3389/fchem.2020.00018
Indigenous content off
Field of Research 091205 Functional Materials
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Grant ID Australian Research Council (CE140100012)
National Health and Medical Research Council (573430)
Australian Nano-Fabrication Facility (ANFF)
Copyright notice ©2020, Quigley, Cornock, Mysore, Foroughi, Kita, Razal, Crook, Moulton, Wallace and Kapsa
Free to Read? Yes
Use Rights Creative Commons Attribution licence
Persistent URL http://hdl.handle.net/10536/DRO/DU:30138032

Document type: Journal Article
Collections: Institute for Frontier Materials
Open Access Collection
GTP Research
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.