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Population genomics of the immune evasion (var) genes of Plasmodium falciparum

Barry, Alyssa, Leliwa-Sytek, A., Tavul, L., Imrie, H., Migot-Nabias, F., Brown, S.M., McVean, G.A.V. and Day, K.P. 2007, Population genomics of the immune evasion (var) genes of Plasmodium falciparum, PLoS Pathogens, vol. 3, no. 3, pp. 1-9, doi: 10.1371/journal.ppat.0030034.


Title Population genomics of the immune evasion (var) genes of Plasmodium falciparum
Author(s) Barry, AlyssaORCID iD for Barry, Alyssa orcid.org/0000-0002-1189-2310
Leliwa-Sytek, A.
Tavul, L.
Imrie, H.
Migot-Nabias, F.
Brown, S.M.
McVean, G.A.V.
Day, K.P.
Journal name PLoS Pathogens
Volume number 3
Issue number 3
Article ID e34
Start page 1
End page 9
Total pages 9
Publisher Public Library of Science
Place of publication San Francisco, Calif.
Publication date 2007-03-16
ISSN 1553-7366
1553-7374
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Microbiology
Parasitology
Virology
PAPUA-NEW-GUINEA
SEVERE CHILDHOOD MALARIA
STRUCTURED VAR GENE
INFECTED ERYTHROCYTES
DIFFERENTIAL EXPRESSION
MICROSATELLITE MARKERS
SEQUENCE ALIGNMENT
CEREBRAL MALARIA
DIVERSITY
RECOMBINATION
Summary Var genes encode the major surface antigen (PfEMP1) of the blood stages of the human malaria parasite Plasmodium falciparum. Differential expression of up to 60 diverse var genes in each parasite genome underlies immune evasion. We compared the diversity of the DBLα domain of var genes sampled from 30 parasite isolates from a malaria endemic area of Papua New Guinea (PNG) and 59 from widespread geographic origins (global). Overall, we obtained over 8,000 quality-controlled DBLα sequences. Within our sampling frame, the global population had a total of 895 distinct DBLα "types" and negligible overlap among repertoires. This indicated that var gene diversity on a global scale is so immense that many genomes would need to be sequenced to capture its true extent. In contrast, we found a much lower diversity in PNG of 185 DBLα types, with an average of approximately 7% overlap among repertoires. While we identify marked geographic structuring, nearly 40% of types identified in PNG were also found in samples from different countries showing a cosmopolitan distribution for much of the diversity. We also present evidence to suggest that recombination plays a key role in maintaining the unprecedented levels of polymorphism found in these immune evasion genes. This population genomic framework provides a cost effective molecular epidemiological tool to rapidly explore the geographic diversity of var genes. © 2007 Barry et al.
Language eng
DOI 10.1371/journal.ppat.0030034
Indigenous content off
Field of Research 0605 Microbiology
1107 Immunology
1108 Medical Microbiology
HERDC Research category C1.1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30138358

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.