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Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system

Kang, Taehee, Park, Chulhun, Meghani, Nileshkumar, Tran, Thao T. D., Tran, Ha Lien Phuong and Lee, Boem-Jin 2020, Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system, Pharmaceutics, vol. 12, no. 6, doi: 10.3390/pharmaceutics12060555.

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Title Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system
Author(s) Kang, Taehee
Park, Chulhun
Meghani, Nileshkumar
Tran, Thao T. D.
Tran, Ha Lien PhuongORCID iD for Tran, Ha Lien Phuong orcid.org/0000-0001-8463-7516
Lee, Boem-Jin
Journal name Pharmaceutics
Volume number 12
Issue number 6
Article ID 555
Total pages 15
Publisher MDPI AG
Place of publication Basel, Switzerland
Publication date 2020-06-30
ISSN 1999-4923
1999-4923
Keyword(s) biomimetic shear stress
cell dynamic environment
cellular uptake
coumarin-6
fattigation-platform NPs
gelatin–oleic conjugate
inhibitory concentration
paclitaxel
self-assembly
Summary Cellular properties and microenvironments, as well as the characteristics of nanoparticles (NPs), affect the cellular uptake and cytotoxic effects of drug-loaded NPs. Since there is fluid flow in the human blood system, fluid flow also affects the drug delivery efficiency of NPs. This study aimed to evaluate the cellular behaviors of drug-loaded soft NPs on A549 cancer cells under different levels of shear stress (0.5, 5, and 50 dynes/cm2) in the biomimetic microfluidic system. The soft self-assembled NPs were formed by the gelatin–oleic conjugate (GOC). The poorly water-soluble coumarin-6 or paclitaxel (PTX) were used as model markers for encapsulation within self-assembled NPs (C-GONs or PTX-GONs, respectively). The cellular uptake of C-GONs was found to be improved with shear-stress dependence. The inhibitory concentration (IC50) of PTX-GONs at 0.5, 5, and 50 dynes/cm2 was 0.106 µg/mL, 0.108 µg/mL, and 0.091 µg/mL, respectively, as compared to 0.138 µg/mL in a static condition. The cell killing efficiency of PTX-GONs was increased in the highest shear stress of 50 dynes/cm2 in the static condition, and other levels of shear stress in dynamic conditions.
Language eng
DOI 10.3390/pharmaceutics12060555
Indigenous content off
Field of Research 1115 Pharmacology and Pharmaceutical Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Copyright notice ©2020, The Authors
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30139601

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.