Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system Kang, Taehee, Park, Chulhun, Meghani, Nileshkumar, Tran, Thao T. D., Tran, Ha Lien Phuong and Lee, Boem-Jin 2020, Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system, Pharmaceutics, vol. 12, no. 6, doi: 10.3390/pharmaceutics12060555. Attached Files Name Description MIMEType Size Downloads Title Shear stress‐dependent targeting efficiency using self‐assembled gelatin–oleic nanoparticles in a biomimetic microfluidic system Author(s) Kang, Taehee Park, Chulhun Meghani, Nileshkumar Tran, Thao T. D. Tran, Ha Lien Phuong orcid.org/0000-0001-8463-7516 Lee, Boem-Jin Journal name Pharmaceutics Volume number 12 Issue number 6 Article ID 555 Total pages 15 Publisher MDPI AG Place of publication Basel, Switzerland Publication date 2020-06-30 ISSN 1999-4923 1999-4923 Keyword(s) biomimetic shear stress cell dynamic environment cellular uptake coumarin-6 fattigation-platform NPs gelatin–oleic conjugate inhibitory concentration paclitaxel self-assembly Summary Cellular properties and microenvironments, as well as the characteristics of nanoparticles (NPs), affect the cellular uptake and cytotoxic effects of drug-loaded NPs. Since there is fluid flow in the human blood system, fluid flow also affects the drug delivery efficiency of NPs. This study aimed to evaluate the cellular behaviors of drug-loaded soft NPs on A549 cancer cells under different levels of shear stress (0.5, 5, and 50 dynes/cm2) in the biomimetic microfluidic system. The soft self-assembled NPs were formed by the gelatin–oleic conjugate (GOC). The poorly water-soluble coumarin-6 or paclitaxel (PTX) were used as model markers for encapsulation within self-assembled NPs (C-GONs or PTX-GONs, respectively). The cellular uptake of C-GONs was found to be improved with shear-stress dependence. The inhibitory concentration (IC50) of PTX-GONs at 0.5, 5, and 50 dynes/cm2 was 0.106 µg/mL, 0.108 µg/mL, and 0.091 µg/mL, respectively, as compared to 0.138 µg/mL in a static condition. The cell killing efficiency of PTX-GONs was increased in the highest shear stress of 50 dynes/cm2 in the static condition, and other levels of shear stress in dynamic conditions. Language eng DOI 10.3390/pharmaceutics12060555 Indigenous content off Field of Research 1115 Pharmacology and Pharmaceutical Sciences HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2020, The Authors Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30139601 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Thu, 02 Jul 2020, 15:47:30 EST Fri, 03 Jul 2020, 05:03:07 EST Mon, 07 Dec 2020, 05:02:43 EST Tue, 08 Dec 2020, 09:45:20 EST Tue, 08 Dec 2020, 09:47:53 EST Tue, 08 Dec 2020, 14:52:48 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 39 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Thu, 02 Jul 2020, 15:47:30 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.