Clinical applicability of methylome sequencing in a pilot study of ischemic stroke individuals Ziemann, Mark, Harikrishnan, K. N., Khurana, Ishant, Kaspi, Antony, Keating, Samuel T., De Silva, Deidre Anne, Moe, Kyaw Thu and El-Osta, Assam 2015, Clinical applicability of methylome sequencing in a pilot study of ischemic stroke individuals, Methods in Next Generation Sequencing, vol. 2, no. 1, pp. 11-22, doi: 10.1515/mngs-2015-0002. Attached Files Name Description MIMEType Size Downloads Title Clinical applicability of methylome sequencing in a pilot study of ischemic stroke individuals Author(s) Ziemann, Mark orcid.org/0000-0002-7688-6974 Harikrishnan, K. N. Khurana, Ishant Kaspi, Antony Keating, Samuel T. De Silva, Deidre Anne Moe, Kyaw Thu El-Osta, Assam Journal name Methods in Next Generation Sequencing Volume number 2 Issue number 1 Start page 11 End page 22 Total pages 12 Publisher De Gruyter Open Place of publication Berlin, Germany Publication date 2015-11-05 ISSN 2084-7173 Keyword(s) homocysteine DNA methylation epigenetics one-carbon metabolism hypertension Summary Despite recent progress in screening for genetic causes of cardiovascular disease using genome-wide association studies, identifying causative polymorphisms has not met initial expectations. This has led to interestin exploring the contribution of non-genetic factors in disease etiology. Elevated plasma homocysteine is an independent risk factor for cardiovascular disease but the mechanism for increased risk remains poorly understood. This study evaluates the clinical applicability of screeningfor genome-wide CpG methylation differences using methyl-CpG binding domain (MBD) protein-enriched genome sequencing (MBD-seq). Peripheral blood genomic DNA methylation in 8 Singaporean-Chinese ischemic stroke patients (4 male, 4 female) was profiled. Differential methylation of genes implicated in hyperhomocysteinemia was observed in males correlating withhomocysteine; namely CBS (cystathionine-beta-synthase) and MTHFR (methylenetetrahydrofolate reductase). In females, hypomethylation of the LDLR (low density lipoprotein receptor) and CELSR1 (cadherin, EGF LAGseven-pass G-type receptor 1) genes were observed in the hypertensive group (2 normal and 2 hypertensive individuals). While the number of clinical samples analysed is small, the findings of this evaluation suggest that MBD-seq is a suitable and sufficiently sensitive technology to determine methylation variability. The results presented warrant an expanded case-control study to determine the pathophysiological implications of DNA methylation for hyper-homocysteinemia. Language eng DOI 10.1515/mngs-2015-0002 Indigenous content off HERDC Research category C1.1 Refereed article in a scholarly journal Copyright notice ©2015, The Authors Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30141378 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Wed, 02 Sep 2020, 13:05:10 EST Thu, 03 Sep 2020, 05:01:04 EST Thu, 03 Sep 2020, 06:03:48 EST Thu, 10 Sep 2020, 09:33:50 EST Thu, 10 Sep 2020, 09:37:29 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 52 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Wed, 02 Sep 2020, 13:05:10 EST

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