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Host-Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model

Pharo, Elizabeth A., Williams, Sinéad M., Boyd, Victoria, Sundaramoorthy, Vinod, Durr, Peter A. and Baker, Michelle L. 2020, Host-Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model, Viruses, vol. 12, no. 6, pp. 1-26, doi: 10.3390/v12060679.

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Title Host-Pathogen Responses to Pandemic Influenza H1N1pdm09 in a Human Respiratory Airway Model
Author(s) Pharo, Elizabeth A.
Williams, Sinéad M.
Boyd, Victoria
Sundaramoorthy, VinodORCID iD for Sundaramoorthy, Vinod orcid.org/0000-0001-6309-8031
Durr, Peter A.
Baker, Michelle L.
Journal name Viruses
Volume number 12
Issue number 6
Article ID 679
Start page 1
End page 26
Total pages 26
Publisher MDPI
Place of publication Basel, Switzerland
Publication date 2020
ISSN 1999-4915
1999-4915
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Virology
epithelium
lung
innate immune system
cytokine
chemokine
antiviral
tight junction
cell death
cytoskeleton
inflammation
SYNDROME CORONAVIRUS INFECTION
EPITHELIAL-CELLS
IN-VITRO
TIGHT JUNCTIONS
INNATE IMMUNITY
BARRIER DYSFUNCTION
ANTIMICROBIAL PEPTIDES
VIRUS-INFECTION
H1N1 2009
Summary The respiratory Influenza A Viruses (IAVs) and emerging zoonotic viruses such as Severe Acute Respiratory Syndrome-Coronavirus-2 (SARS-CoV-2) pose a significant threat to human health. To accelerate our understanding of the host–pathogen response to respiratory viruses, the use of more complex in vitro systems such as normal human bronchial epithelial (NHBE) cell culture models has gained prominence as an alternative to animal models. NHBE cells were differentiated under air-liquid interface (ALI) conditions to form an in vitro pseudostratified epithelium. The responses of well-differentiated (wd) NHBE cells were examined following infection with the 2009 pandemic Influenza A/H1N1pdm09 strain or following challenge with the dsRNA mimic, poly(I:C). At 30 h postinfection with H1N1pdm09, the integrity of the airway epithelium was severely impaired and apical junction complex damage was exhibited by the disassembly of zona occludens-1 (ZO-1) from the cell cytoskeleton. wdNHBE cells produced an innate immune response to IAV-infection with increased transcription of pro- and anti-inflammatory cytokines and chemokines and the antiviral viperin but reduced expression of the mucin-encoding MUC5B, which may impair mucociliary clearance. Poly(I:C) produced similar responses to IAV, with the exception of MUC5B expression which was more than 3-fold higher than for control cells. This study demonstrates that wdNHBE cells are an appropriate ex-vivo model system to investigate the pathogenesis of respiratory viruses.
Language eng
DOI 10.3390/v12060679
Indigenous content off
Field of Research 0605 Microbiology
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30141712

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
Open Access Collection
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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.