Investigating both mucosal immunity and microbiota in response to gut enteritis in yellowtail kingfish Legrand, Thibault P. R. A., Wynne, James W., Weyrich, Laura S. and Oxley, Andrew P. A. 2020, Investigating both mucosal immunity and microbiota in response to gut enteritis in yellowtail kingfish, Microorganisms, vol. 8, no. 9, Special Issue Host–Microbe Interactions in Animal/Human Health and Disease, pp. 1-17, doi: 10.3390/microorganisms8091267. Attached Files Name Description MIMEType Size Downloads Title Investigating both mucosal immunity and microbiota in response to gut enteritis in yellowtail kingfish Author(s) Legrand, Thibault P. R. A. Wynne, James W. Weyrich, Laura S. Oxley, Andrew P. A. orcid.org/0000-0002-6056-7602 Journal name Microorganisms Volume number 8 Issue number 9 Season Special Issue Host–Microbe Interactions in Animal/Human Health and Disease Article ID 1267 Start page 1 End page 17 Total pages 17 Publisher MDPI Place of publication Basel, Switzerland Publication date 2020 ISSN 2076-2607 2076-2607 Keyword(s) aquaculture fish gut health immunity microbiota mucosa skin Summary The mucosal surfaces of fish play numerous roles including, but not limited to, protection against pathogens, nutrient digestion and absorption, excretion of nitrogenous wastes and osmotic regulation. During infection or disease, these surfaces act as the first line of defense, where the mucosal immune system interacts closely with the associated microbiota to maintain homeostasis. This study evaluated microbial changes across the gut and skin mucosal surfaces in yellowtail kingfish displaying signs of gut inflammation, as well as explored the host gene expression in these tissues in order to improve our understanding of the underlying mechanisms that contribute to the emergence of these conditions. For this, we obtained and analyzed 16S rDNA and transcriptomic (RNA-Seq) sequence data from the gut and skin mucosa of fish exhibiting different health states (i.e., healthy fish and fish at the early and late stages of enteritis). Both the gut and skin microbiota were perturbed by the disease. More specifically, the gastrointestinal microbiota of diseased fish was dominated by an uncultured Mycoplasmataceae sp., and fish at the early stage of the disease showed a significant loss of diversity in the skin. Using transcriptomics, we found that only a few genes were significantly differentially expressed in the gut. In contrast, gene expression in the skin differed widely between health states, in particular in the fish at the late stage of the disease. These changes were associated with several metabolic pathways that were differentially expressed and reflected a weakened host. Altogether, this study highlights the sensitivity of the skin mucosal surface in response to gut inflammation. Language eng DOI 10.3390/microorganisms8091267 Indigenous content off HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30142588 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Tue, 22 Sep 2020, 12:36:21 EST Wed, 23 Sep 2020, 05:00:51 EST Wed, 23 Sep 2020, 06:04:15 EST Wed, 23 Sep 2020, 14:56:11 EST Wed, 23 Sep 2020, 14:58:24 EST Wed, 23 Sep 2020, 14:59:10 EST Wed, 23 Sep 2020, 15:13:03 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 18 Abstract Views, 1 File Downloads  -  Detailed Statistics Created: Tue, 22 Sep 2020, 12:36:21 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.