Impact of in vitro embryo culture and transfer on blood pressure regulation in the adolescent lamb

Padhee, Monalisa, McMillen, I Caroline, Zhang, Song, MacLaughlin, Severence M, Armitage, James A, Head, Geoffrey A, Darby, Jack RT, Kelly, Jennifer M, Rudiger, Skye R, Kleemann, David O, Walker, Simon K and Morrison, Janna L 2020, Impact of in vitro embryo culture and transfer on blood pressure regulation in the adolescent lamb, Journal of developmental origins of health and disease, pp. 1-7, doi: 10.1017/s2040174420001014.

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Title Impact of in vitro embryo culture and transfer on blood pressure regulation in the adolescent lamb
Author(s) Padhee, Monalisa
McMillen, I Caroline
Zhang, Song
MacLaughlin, Severence M
Armitage, James AORCID iD for Armitage, James A orcid.org/0000-0002-3762-0911
Head, Geoffrey A
Darby, Jack RT
Kelly, Jennifer M
Rudiger, Skye R
Kleemann, David O
Walker, Simon K
Morrison, Janna L
Journal name Journal of developmental origins of health and disease
Start page 1
End page 7
Total pages 7
Publisher Cambridge University Press
Place of publication Cambridge, Eng.
Publication date 2020-11-13
ISSN 2040-1744
2040-1752
Keyword(s) DOHaD
In vitro embryo culture
artificial reproductive technologies
baroreflex
cardiac
embryo transfer
hypertension
Summary Nutrition during the periconceptional period influences postnatal cardiovascular health. We determined whether in vitro embryo culture and transfer, which are manipulations of the nutritional environment during the periconceptional period, dysregulate postnatal blood pressure and blood pressure regulatory mechanisms. Embryos were either transferred to an intermediate recipient ewe (ET) or cultured in vitro in the absence (IVC) or presence of human serum (IVCHS) and a methyl donor (IVCHS+M) for 6 days. Basal blood pressure was recorded at 19–20 weeks after birth. Mean arterial pressure (MAP) and heart rate (HR) were measured before and after varying doses of phenylephrine (PE). mRNA expression of signaling molecules involved in blood pressure regulation was measured in the renal artery. Basal MAP did not differ between groups. Baroreflex sensitivity, set point, and upper plateau were also maintained in all groups after PE stimulation. Adrenergic receptors alpha-1A (αAR1A), alpha-1B (αAR1B), and angiotensin II receptor type 1 (AT1R) mRNA expression were not different from controls in the renal artery. These results suggest there is no programmed effect of ET or IVC on basal blood pressure or the baroreflex control mechanisms in adolescence, but future studies are required to determine the impact of ET and IVC on these mechanisms later in the life course when developmental programming effects may be unmasked by age.
Language eng
DOI 10.1017/s2040174420001014
Indigenous content off
Field of Research 11 Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Persistent URL http://hdl.handle.net/10536/DRO/DU:30145478

Document type: Journal Article
Collections: Faculty of Health
School of Medicine
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