Reduction of hippocampal IL-6 levels in LPS-injected rats following acute exendin-4 treatment Géa, Luiza P., da Rosa, Eduardo D., Panizzutti, Bruna S., de Aguiar, Érica Z., de Oliveira, Larissa F., Ferrari, Pamela, Piato, Angelo, Gomez, Rosane, Colombo, Rafael and Rosa, Adriane R. 2020, Reduction of hippocampal IL-6 levels in LPS-injected rats following acute exendin-4 treatment, Naunyn-Schmiedeberg's Archives of Pharmacology, vol. 393, no. 7, pp. 1303-1311, doi: 10.1007/s00210-020-01867-5. Attached Files Name Description MIMEType Size Downloads Title Reduction of hippocampal IL-6 levels in LPS-injected rats following acute exendin-4 treatment Author(s) Géa, Luiza P. da Rosa, Eduardo D. Panizzutti, Bruna S. de Aguiar, Érica Z. de Oliveira, Larissa F. Ferrari, Pamela Piato, Angelo Gomez, Rosane Colombo, Rafael Rosa, Adriane R. Journal name Naunyn-Schmiedeberg's Archives of Pharmacology Volume number 393 Issue number 7 Start page 1303 End page 1311 Total pages 9 Publisher Springer Place of publication Heidelberg, Germany Publication date 2020-07-01 ISSN 0028-1298 1432-1912 Keyword(s) Brain-derived neurotrophic factor Glucagon-like peptide 1 Inflammation Interleukin-6 Lipopolysaccharide Thiobarbituric acid reactive substances Summary Preclinical evidence on the role of glucagon-like peptide-1 receptor (GLP-1r) agonists in the brain led to an increased interest in repurposing these compounds as a therapy for central nervous system (CNS) disorders and associated comorbidities. We aimed to investigate the neuroprotective effects of acute treatment with exendin (EX)-4, a GLP-1r agonist, in an animal model of inflammation. We evaluated the effect of different doses of EX-4 on inflammatory, neurotrophic, and oxidative stress parameters in the hippocampus and serum of lipopolysaccharide (LPS)-injected animals. Male Wistar rats were injected with LPS (0.25 mg/kg i.p.) and treated with different doses of EX-4 (0.1, 0.3, or 0.5 μg/kg i.p.). Sickness behavior was assessed by locomotor activity and body weight, and depressive-like behavior was also evaluated using forced swim test (FST). Brain-derived neurotrophic factor (BDNF), thiobarbituric acid reactive species (TBARS), and interleukin (IL)-6 were quantified in the serum and hippocampus. Glycemia was also analyzed pre- and post-EX-4 treatment. LPS groups exhibited decreased frequency of crossing and reduced body weight (p < 0.001), while alterations on FST were not observed. The higher dose of EX-4 reduced IL-6 in the hippocampus of LPS-injected animals (p = 0.018), and EX-4 per se reduced TBARS serum levels with a modest antioxidant effect in the LPS groups (p ≤ 0.005). BDNF hippocampal levels seemed to be increased in the LPS+EX-4 0.5 group compared with LPS+Saline (p > 0.05). Our study provides evidence on acute anti-inflammatory effects of EX-4 in the hippocampus of rats injected with LPS, contributing to future studies on repurposing compounds with potential neuroprotective properties. Language eng DOI 10.1007/s00210-020-01867-5 Indigenous content off Field of Research 1115 Pharmacology and Pharmaceutical Sciences 1116 Medical Physiology HERDC Research category C1 Refereed article in a scholarly journal Copyright notice ©2020, Springer-Verlag GmbH Germany Persistent URL http://hdl.handle.net/10536/DRO/DU:30146071 Document type: Journal Article Collection: Faculty of Health Related Links Link Description Connect to published version Go to link with your DU access privileges     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Versions Version Filter Type Thu, 03 Dec 2020, 13:31:56 EST Thu, 03 Dec 2020, 14:01:09 EST Thu, 03 Dec 2020, 14:13:50 EST Mon, 07 Dec 2020, 05:05:15 EST Wed, 09 Dec 2020, 14:38:52 EST Tue, 15 Dec 2020, 06:10:01 EST Tue, 15 Dec 2020, 06:13:38 EST Tue, 15 Dec 2020, 15:05:57 EST Sat, 09 Jan 2021, 01:36:46 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 34 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Thu, 03 Dec 2020, 13:31:56 EST

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