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Differential level of RXFP3 expression in dopaminergic neurons within the arcuate nucleus, dorsomedial hypothalamus and ventral tegmental area of RXFP3-Cre/tdTomato mice

Voglsanger, Lara M, Read, Justin, Ch’ng, Sarah S, Zhang, Cary, Eraslan, Izel M, Gray, Laura, Rivera, Leni R, Hamilton, Lee D, Williams, Richard, Gundlach, Andrew L and Smith, Craig M 2021, Differential level of RXFP3 expression in dopaminergic neurons within the arcuate nucleus, dorsomedial hypothalamus and ventral tegmental area of RXFP3-Cre/tdTomato mice, Frontiers in neuroscience, vol. 14, pp. 1-10, doi: 10.3389/fnins.2020.594818.

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Title Differential level of RXFP3 expression in dopaminergic neurons within the arcuate nucleus, dorsomedial hypothalamus and ventral tegmental area of RXFP3-Cre/tdTomato mice
Author(s) Voglsanger, Lara M
Read, JustinORCID iD for Read, Justin orcid.org/0000-0003-3790-0873
Ch’ng, Sarah S
Zhang, Cary
Eraslan, Izel M
Gray, LauraORCID iD for Gray, Laura orcid.org/0000-0002-7903-5796
Rivera, Leni R
Hamilton, Lee D
Williams, RichardORCID iD for Williams, Richard orcid.org/0000-0003-3831-8738
Gundlach, Andrew L
Smith, Craig MORCID iD for Smith, Craig M orcid.org/0000-0002-2894-2433
Journal name Frontiers in neuroscience
Volume number 14
Article ID 594818
Start page 1
End page 10
Total pages 10
Publisher Frontiers Research Foundation
Place of publication Lausanne, Switzerland
Publication date 2021-01-06
ISSN 1662-4548
1662-453X
Keyword(s) Science & Technology
Life Sciences & Biomedicine
Neurosciences
Neurosciences & Neurology
RXFP3
relaxin-3
dopamine
tyrosine hydroxylase
arcuate nucleus
dorsomedial hypothalamus
Summary RXFP3 (relaxin-family peptide 3 receptor) is the cognate G-protein-coupled receptor for the neuropeptide, relaxin-3. RXFP3 is expressed widely throughout the brain, including the hypothalamus, where it has been shown to modulate feeding behavior and neuroendocrine activity in rodents. In order to better characterize its potential mechanisms of action, this study determined whether RXFP3 is expressed by dopaminergic neurons within the arcuate nucleus (ARC) and dorsomedial hypothalamus (DMH), in addition to the ventral tegmental area (VTA). Neurons that express RXFP3 were visualized in coronal brain sections from RXFP3-Cre/tdTomato mice, which express the tdTomato fluorophore within RXFP3-positive cells, and dopaminergic neurons in these areas were visualized by simultaneous immunohistochemical detection of tyrosine hydroxylase-immunoreactivity (TH-IR). Approximately 20% of ARC neurons containing TH-IR coexpressed tdTomato fluorescence, suggesting that RXFP3 can influence the dopamine pathway from the ARC to the pituitary gland that controls prolactin release. The ability of prolactin to reduce leptin sensitivity and increase food consumption therefore represents a potential mechanism by which RXFP3 activation influences feeding. A similar proportion of DMH neurons containing TH-IR expressed RXFP3-related tdTomato fluorescence, consistent with a possible RXFP3-mediated regulation of stress and neuroendocrine circuits. In contrast, RXFP3 was barely detected within the VTA. TdTomato signal was absent from the ARC and DMH in sections from Rosa26-tdTomato mice, suggesting that the cells identified in RXFP3-Cre/tdTomato mice expressed authentic RXFP3-related tdTomato fluorescence. Together, these findings identify potential hypothalamic mechanisms through which RXFP3 influences neuroendocrine control of metabolism, and further highlight the therapeutic potential of targeting RXFP3 in feeding-related disorders.
Language eng
DOI 10.3389/fnins.2020.594818
Indigenous content off
Field of Research 1109 Neurosciences
1701 Psychology
1702 Cognitive Sciences
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30146528

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.