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An in vitro ovarian explant culture system to examine sex change in a hermaphroditic fish

Goikoetxea, Alexander, Damsteegt, Erin L, Todd, Erica V, McNaughton, Andrew, Gemmell, Neil J and Lokman, P Mark 2020, An in vitro ovarian explant culture system to examine sex change in a hermaphroditic fish, PeerJ, vol. 8, pp. 1-13, doi: 10.7717/peerj.10323.

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Title An in vitro ovarian explant culture system to examine sex change in a hermaphroditic fish
Author(s) Goikoetxea, Alexander
Damsteegt, Erin L
Todd, Erica V
McNaughton, Andrew
Gemmell, Neil J
Lokman, P Mark
Journal name PeerJ
Volume number 8
Article ID e10323
Start page 1
End page 13
Total pages 13
Publisher PeerJ Inc.
Place of publication Corte Madera, Calif.
Publication date 2020-11-11
ISSN 2167-8359
Keyword(s) Organ culture
Sex change
Previtellogenic oocyte
Cortisol
Spotty wrasse
Summary Many teleost fishes undergo natural sex change, and elucidating the physiological and molecular controls of this process offers unique opportunities not only to develop methods of controlling sex in aquaculture settings, but to better understand vertebrate sexual development more broadly. Induction of sex change in some sequentially hermaphroditic or gonochoristic fish can be achieved in vivo through social manipulation, inhibition of aromatase activity, or steroid treatment. However, the induction of sex change in vitro has been largely unexplored. In this study, we established an in vitro culture system for ovarian explants in serum-free medium for a model sequential hermaphrodite, the New Zealand spotty wrasse (Notolabrus celidotus). This culture technique enabled evaluating the effect of various treatments with 17β-estradiol (E2), 11-ketotestosterone (11KT) or cortisol (CORT) on spotty wrasse ovarian architecture for 21 days. A quantitative approach to measuring the degree of ovarian atresia within histological images was also developed, using pixel-based machine learning software. Ovarian atresia likely due to culture was observed across all treatments including no-hormone controls, but was minimised with treatment of at least 10 ng/mL E2. Neither 11KT nor CORT administration induced proliferation of spermatogonia (i.e., sex change) in the cultured ovaries indicating culture beyond 21 days may be needed to induce sex change in vitro. The in vitro gonadal culture and analysis systems established here enable future studies investigating the paracrine role of sex steroids, glucocorticoids and a variety of other factors during gonadal sex change in fish.
Language eng
DOI 10.7717/peerj.10323
Indigenous content off
Field of Research 06 Biological Sciences
11 Medical and Health Sciences
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30146533

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.