RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines Monroe, Jerry D., Moolani, Satya A., Irihamye, Elvin N., Speed, Joshua S., Gibert, Yann and Smith, Michael E. 2020, RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines, Cells, vol. 9, no. 12, pp. 1-17, doi: 10.3390/cells9122637. Attached Files Name Description MIMEType Size Downloads Title RNA-Seq analysis of cisplatin and the monofunctional platinum(II) complex, phenanthriplatin, in A549 non-small cell lung cancer and IMR90 lung fibroblast cell lines Author(s) Monroe, Jerry D. Moolani, Satya A. Irihamye, Elvin N. Speed, Joshua S. Gibert, Yann Smith, Michael E. Journal name Cells Volume number 9 Issue number 12 Article ID 2637 Start page 1 End page 17 Total pages 17 Publisher MDPI AG Place of publication Basel, Switzerland Publication date 2020-12-08 ISSN 2073-4409 Keyword(s) cancer cisplatin monofunctional platinum(II) complex next generation sequencing pathway analysis Summary Phenanthriplatin is a new monofunctional platinum(II) complex that binds only one strand of DNA and acts by blocking gene transcription, but its effect on gene regulation has not been characterized relative to the traditional platinum-based complex, cisplatin. A549 non-small cell lung cancer and IMR90 lung fibroblast cells were treated with cisplatin, phenanthriplatin, or a control and then their RNA transcripts were subjected to next generation sequencing analysis. DESeq2 and CuffDiff2 were used to identify up- and downregulated genes and Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases were used to identify pathways and functions. We found that phenanthriplatin may regulate the genes GPRC5a, TFF1, and TNFRSF10D, which act through p53 to control apoptosis, differently or to a greater extent than cisplatin, and that it, unlike cisplatin, could upregulate ATP5MD, a gene which signals through the Wnt/β catenin pathway. Furthermore, phenanthriplatin caused unique or enhanced effects compared to cisplatin on genes regulating the cytoskeleton, cell migration, and proliferation, e.g., AGAP1, DIAPH2, GDF15, and THSD1 (p < 0.05; q < 0.05). Phenanthriplatin may modulate some oncogenes differently than cisplatin potentially leading to improved clinical outcome, but this monofunctional complex should be carefully matched with cancer gene data to be successfully applied in chemotherapy. Language eng DOI 10.3390/cells9122637 Indigenous content off HERDC Research category C1 Refereed article in a scholarly journal Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30146694 Document type: Journal Article Collections: Faculty of Health School of Medicine Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Tue, 12 Jan 2021, 13:10:47 EST Wed, 13 Jan 2021, 04:02:59 EST Sat, 16 Jan 2021, 05:13:39 EST Mon, 08 Feb 2021, 13:31:28 EST Mon, 08 Feb 2021, 13:34:15 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 9 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Tue, 12 Jan 2021, 13:10:47 EST

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