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The Pontastacus leptodactylus (Astacidae) repeatome provides insight into genome evolution and reveals remarkable diversity of satellite DNA

Boštjančić, Ljudevit Luka, Bonassin, Lena, Anušić, Lucija, Lovrenčić, Leona, Besendorfer, Višnja, Maguire, Ivana, Grandjean, Frederic, Austin, Christopher M, Greve, Carola, Hamadou, Alexander B and Mlinarec, Jelena 2021, The Pontastacus leptodactylus (Astacidae) repeatome provides insight into genome evolution and reveals remarkable diversity of satellite DNA, Frontiers in genetics, vol. 11, pp. 1-16, doi: 10.3389/fgene.2020.611745.

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Title The Pontastacus leptodactylus (Astacidae) repeatome provides insight into genome evolution and reveals remarkable diversity of satellite DNA
Author(s) Boštjančić, Ljudevit Luka
Bonassin, Lena
Anušić, Lucija
Lovrenčić, Leona
Besendorfer, Višnja
Maguire, Ivana
Grandjean, Frederic
Austin, Christopher MORCID iD for Austin, Christopher M orcid.org/0000-0003-1848-6267
Greve, Carola
Hamadou, Alexander B
Mlinarec, Jelena
Journal name Frontiers in genetics
Volume number 11
Article ID 611745
Start page 1
End page 16
Total pages 16
Publisher Frontiers Media
Place of publication Lausanne, Switzerland
Publication date 2021-01
ISSN 1664-8021
1664-8021
Keyword(s) FISH
genome size
interstitial telomeric repeats
karyotype
narrow-clawed crayfish
(peri)centromeric heterochromatin
Summary Pontastacus leptodactylus is a native European crayfish species found in both freshwater and brackish environments. It has commercial importance for fisheries and aquaculture industries. Up till now, most studies concerning P. leptodactylus have focused onto gaining knowledge about its phylogeny and population genetics. However, little is known about the chromosomal evolution and genome organization of this species. Therefore, we performed clustering analysis of a low coverage genomic dataset to identify and characterize repetitive DNA in the P. leptodactylus genome. In addition, the karyogram of P. leptodactylus (2n = 180) is presented here for the first time consisting of 75 metacentric, 14 submetacentric, and a submetacentric/metacentric heteromorphic chromosome pair. We determined the genome size to be at ~18.7 gigabase pairs. Repetitive DNA represents about 54.85% of the genome. Satellite DNA repeats are the most abundant type of repetitive DNA, making up to ~28% of the total amount of repetitive elements, followed by the Ty3/Gypsy retroelements (~15%). Our study established a surprisingly high diversity of satellite repeats in P. leptodactylus. The genome of P. leptodactylus is by far the most satellite-rich genome discovered to date with 258 satellite families described. Of the five mapped satellite DNA families on chromosomes, PlSAT3-411 co-localizes with the AT-rich DAPI positive probable (peri)centromeric heterochromatin on all chromosomes, while PlSAT14-79 co-localizes with the AT-rich DAPI positive (peri)centromeric heterochromatin on one chromosome and is also located subterminally and intercalary on some chromosomes. PlSAT1-21 is located intercalary in the vicinity of the (peri)centromeric heterochromatin on some chromosomes, while PlSAT6-70 and PlSAT7-134 are located intercalary on some P. leptodactylus chromosomes. The FISH results reveal amplification of interstitial telomeric repeats (ITRs) in P. leptodactylus. The prevalence of repetitive elements, especially the satellite DNA repeats, may have provided a driving force for the evolution of the P. leptodactylus genome.
Language eng
DOI 10.3389/fgene.2020.611745
Indigenous content off
Field of Research 0604 Genetics
1103 Clinical Sciences
1801 Law
HERDC Research category C1 Refereed article in a scholarly journal
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30148128

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.