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Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway

Li, L, Zhang, Y, Wang, M, Zhou, J, Zhang, Q, Yang, Wenrong, Li, Y and Yan, F 2021, Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway, Frontiers in Bioengineering and Biotechnology, vol. 9, pp. 1-12, doi: 10.3389/fbioe.2021.631191.

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Title Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway
Author(s) Li, L
Zhang, Y
Wang, M
Zhou, J
Zhang, Q
Yang, WenrongORCID iD for Yang, Wenrong orcid.org/0000-0001-8815-1951
Li, Y
Yan, F
Journal name Frontiers in Bioengineering and Biotechnology
Volume number 9
Article ID 631191
Start page 1
End page 12
Total pages 12
Publisher Frontiers Media SA
Place of publication Lausanne, Switzerland
Publication date 2021-01
ISSN 2296-4185
2296-4185
Keyword(s) gold nanoparticles
human β-defensin 3 gene
osteogenic differentiation
p38 MAPK pathway
periodontal ligament cells
Summary Periodontitis is a chronic inflammatory disease with plaques as the initiating factor, which will induce the destruction of periodontal tissues. Numerous studies focused on how to obtain periodontal tissue regeneration in inflammatory environments. Previous studies have reported adenovirus-mediated human β-defensin 3 (hBD3) gene transfer could potentially enhance the osteogenic differentiation of human periodontal ligament cells (hPDLCs) and bone repair in periodontitis. Gold nanoparticles (AuNPs), the ideal inorganic nanomaterials in biomedicine applications, were proved to have synergetic effects with gene transfection. To further observe the potential promoting effects, AuNPs were added to the transfected cells. The results showed the positive effects of osteogenic differentiation while applying AuNPs into hPDLCs transfected by adenovirus encoding hBD3 gene. In vivo, after rat periodontal ligament cell (rPDLC) transplantation into SD rats with periodontitis, AuNPs combined hBD3 gene modification could also promote periodontal regeneration. The p38 mitogen-activated protein kinase (MAPK) pathway was demonstrated to potentially regulate both the in vitro and in vivo processes. In conclusion, AuNPs can promote the osteogenic differentiation of hBD3 gene-modified hPDLCs and periodontal regeneration via the p38 MAPK pathway.
Language eng
DOI 10.3389/fbioe.2021.631191
Indigenous content off
Field of Research 0699 Other Biological Sciences
0903 Biomedical Engineering
1004 Medical Biotechnology
HERDC Research category C1 Refereed article in a scholarly journal
ERA Research output type C Journal article
Free to Read? Yes
Persistent URL http://hdl.handle.net/10536/DRO/DU:30148214

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Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.