Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway Li, L, Zhang, Y, Wang, M, Zhou, J, Zhang, Q, Yang, Wenrong, Li, Y and Yan, F 2021, Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway, Frontiers in Bioengineering and Biotechnology, vol. 9, pp. 1-12, doi: 10.3389/fbioe.2021.631191. Attached Files Name Description MIMEType Size Downloads Title Gold Nanoparticles Combined Human β-Defensin 3 Gene-Modified Human Periodontal Ligament Cells Alleviate Periodontal Destruction via the p38 MAPK Pathway Author(s) Li, L Zhang, Y Wang, M Zhou, J Zhang, Q Yang, Wenrong orcid.org/0000-0001-8815-1951 Li, Y Yan, F Journal name Frontiers in Bioengineering and Biotechnology Volume number 9 Article ID 631191 Start page 1 End page 12 Total pages 12 Publisher Frontiers Media SA Place of publication Lausanne, Switzerland Publication date 2021-01 ISSN 2296-4185 2296-4185 Keyword(s) gold nanoparticles human β-defensin 3 gene osteogenic differentiation p38 MAPK pathway periodontal ligament cells Summary Periodontitis is a chronic inflammatory disease with plaques as the initiating factor, which will induce the destruction of periodontal tissues. Numerous studies focused on how to obtain periodontal tissue regeneration in inflammatory environments. Previous studies have reported adenovirus-mediated human β-defensin 3 (hBD3) gene transfer could potentially enhance the osteogenic differentiation of human periodontal ligament cells (hPDLCs) and bone repair in periodontitis. Gold nanoparticles (AuNPs), the ideal inorganic nanomaterials in biomedicine applications, were proved to have synergetic effects with gene transfection. To further observe the potential promoting effects, AuNPs were added to the transfected cells. The results showed the positive effects of osteogenic differentiation while applying AuNPs into hPDLCs transfected by adenovirus encoding hBD3 gene. In vivo, after rat periodontal ligament cell (rPDLC) transplantation into SD rats with periodontitis, AuNPs combined hBD3 gene modification could also promote periodontal regeneration. The p38 mitogen-activated protein kinase (MAPK) pathway was demonstrated to potentially regulate both the in vitro and in vivo processes. In conclusion, AuNPs can promote the osteogenic differentiation of hBD3 gene-modified hPDLCs and periodontal regeneration via the p38 MAPK pathway. Language eng DOI 10.3389/fbioe.2021.631191 Indigenous content off Field of Research 0699 Other Biological Sciences 0903 Biomedical Engineering 1004 Medical Biotechnology HERDC Research category C1 Refereed article in a scholarly journal ERA Research output type C Journal article Free to Read? Yes Persistent URL http://hdl.handle.net/10536/DRO/DU:30148214 Document type: Journal Article Collections: Faculty of Science, Engineering and Built Environment School of Life and Environmental Sciences Open Access Collection Related Links Link Description Link to full-text (open access)     Unless expressly stated otherwise, the copyright for items in DRO is owned by the author, with all rights reserved. Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au. Versions Version Filter Type Mon, 22 Feb 2021, 08:04:30 EST Tue, 23 Feb 2021, 04:01:38 EST Tue, 23 Feb 2021, 07:10:05 EST Thu, 25 Feb 2021, 15:29:23 EST Fri, 26 Feb 2021, 05:04:22 EST Fri, 26 Feb 2021, 14:05:18 EST Fri, 26 Feb 2021, 14:06:36 EST Fri, 26 Feb 2021, 14:07:22 EST Sat, 13 Mar 2021, 01:31:12 EST Filtered Full Citation counts:   Cited 0 times in TR Web of Science Cited 0 times in Scopus Search Google Scholar Access Statistics: 19 Abstract Views, 0 File Downloads  -  Detailed Statistics Created: Thu, 25 Feb 2021, 15:29:23 EST

Every reasonable effort has been made to ensure that permission has been obtained for items included in DRO. If you believe that your rights have been infringed by this repository, please contact drosupport@deakin.edu.au.