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Estimating nephron number in the developing kidney using the physical disector/fractionator combination

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posted on 2012-01-01, 00:00 authored by L Cullen-McEwen, James ArmitageJames Armitage, J Nyengaard, J Bertram
Over the last decade the development of new molecular biology tools, advanced microscopy, live imaging and systems biology approaches have revolutionized our conception of how embryonic development proceeds. One fundamental aspect of development biology is the concept of morphogenesis: understanding how a group of multipotent cells organize and differentiate into a complex organ. In Kidney Development: Methods and Protocols, expert researchers in the field detail different approaches to tackle kidney development. These approaches include culture and live imaging aspects of kidney development, analyzing the 3-dimensional aspects of branching morphogenesis as well as nephrogenesis, manipulation of the gene/protein expression during kidney development as well as in the adult kidney, and how to assess kidney malformation and disease. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Kidney Development: Methods and Protocols seeks to aid scientists in the further study of the process of morphogenesis which is fundamental important not only for studying developmental biology but also for regenerative medicine.

History

Title of book

Kidney development: methods and protocols

Series

Methods in molecular biology 886

Chapter number

10

Pagination

109 - 119

Publisher

Springer Science & Business Media

Place of publication

Dordrecht, The Netherlands

ISBN-13

9781617798511

ISBN-10

1617798517

Language

eng

Publication classification

B1.1 Book chapter; C Journal article

Copyright notice

2012, Springer

Extent

35

Editor/Contributor(s)

O Michos

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