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Functional analysis of circular RNAs

Version 2 2024-06-06, 09:02
Version 1 2019-03-14, 12:41
chapter
posted on 2024-06-06, 09:02 authored by Shanmugapriya, Hisham Alkatib Huda, Soundararajan Vijayarathna, Chern Ein Oon, Yeng Chen, Jagat R Kanwar, Mei Li Ng, Sreenivasan Sasidharan
Circular RNAs characterize a class of widespread and diverse endogenous RNAs which are non-coding RNAs that are made by back-splicing events and have covalently closed loops with no polyadenylated tails. Various indications specify that circular RNAs (circRNAs) are plentiful in the human transcriptome. However, their participation in biological processes remains mostly undescribed. To date thousands of circRNAs have been revealed in organisms ranging from Drosophila melanogaster to Homo sapiens. Functional studies specify that these transcripts control expression of protein-coding linear transcripts and thus encompass a key component of gene expression regulation. This chapter provide a comprehensive overview on functional validation of circRNAs. Furthermore, we discuss the recent modern methodologies for the functional validation of circRNAs such as RNA interference (RNAi) gene silencing assay, luciferase reporter assays, circRNA gain-of-function investigation via overexpression of circular transcript assay, RT-q-PCR quantification, and other latest applicable assays. The methods described in this chapter are demonstrated on the cellular model.

History

Volume

1087

Pagination

95-105

ISSN

0065-2598

Language

eng

Publication classification

B1 Book chapter

Copyright notice

2018, Springer Nature Singapore Pte Ltd.

Editor/Contributor(s)

Xiao J

Publisher

Springer

Place of publication

Singapore

Title of book

Circular RNAs: biogenesis and functions

Series

Advances in experimental medicine and biology

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