Sex identification of ancient DNA samples using a microfluidic device
Version 2 2024-06-13, 11:08Version 2 2024-06-13, 11:08
Version 1 2018-10-23, 15:38Version 1 2018-10-23, 15:38
chapter
posted on 2024-06-13, 11:08 authored by KJ Shaw, KA Brown, TA Brown, SJ Haswell© Springer Science+Business Media New York 2015. Ancient DNA is the name given to the degraded, fragmented, and chemically damaged biomolecules that can be recovered from archaeological remains of plants, animals, and humans. Where ancient human DNA has survived at archaeological sites, it can give valuable information and is especially useful for its potential to identify kinship, population affinities, pathogens, and biological sex. Here, we describe the operation of a microfluidic device for the sex identification of ancient DNA samples using an efficient sample handling process. DNA is extracted from powdered bone samples and abasic sites labeled with biotin. Streptavidincoated superparamagnetic particles are used to isolate the labeled DNA prior to amplification of the Amelogenin sex marker.
History
Volume
1274Pagination
93-98Publisher DOI
ISSN
1064-3745Publication classification
X Not reportable, BN.1 Other book chapter, or book chapter not attributed to DeakinPublisher
Humana PressPlace of publication
New York, N.Y.Title of book
Microchip Capillary Electrophoresis ProtocolsUsage metrics
Categories
Licence
Exports
RefWorksRefWorks
BibTeXBibTeX
Ref. managerRef. manager
EndnoteEndnote
DataCiteDataCite
NLMNLM
DCDC