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Agarose for mechanical control of self-assembling peptide systems

conference contribution
posted on 2019-01-01, 00:00 authored by K Firipis, Mitchell Boyd-Moss, A Quigley, D Nisbet, Richard WilliamsRichard Williams
Statement of Purpose: Advances in understanding of extracellular matrix-cell interactions has informed a new generation of rationally designed biomaterials [1]. Biochemical, structural and mechanical cues, present in the native extracellular matrix drive cell behaviour. Fmoc-FRGDF/PHSRN is a rationally designed biomaterial, a self-assembling peptide that relies on π-π stacking to form a structurally fibrous network; mimicking the structure of the extracellular matrix. Additionally, Fmoc-FRGDF/PHSRN is based on key cell attachment motifs (RGD and PHSRN) from the extracellular matrix protein fibronectin. Fmoc-FRGDF/PHSRN has been shown to promote cell attachment in vitro due to its structural and biochemical similarities to the native extracellular matrix [2]. A key limitation of Fmoc-FRGDF/PHSRN is that the mechanical properties, and therefore applicability to various cell types mechanbiology requirements and bioprinting, cannot be modulated. By adding an inert polysaccharide, namely Agarose in varying concentrations, the mechanical properties are modulated without disrupting the biochemical and structural properties or self-assembling gelation process.

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Volume

40

Pagination

698 - ?

ISSN

1526-7547

ISBN-13

9781510883901

Publication classification

E3 Extract of paper

Title of proceedings

Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium

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