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DNA extraction, using carrier RNA, integrated with agarose gel-based polymerase chain reaction in a microfluidic device
conference contributionposted on 2023-02-05, 22:41 authored by K J Shaw, J Oakley, P T Docker, C E Dyer, J Greenman, G M Greenway, Stephen Haswell
This presentation reports an integrated DNA extraction and amplification device based on electro-osmotic pumping (EOP). Solid-phase DNA extraction is performed on a thermally activated silica-based monolith and carrier RNA is included in the chaotropic salt solution to increase the efficiency of the DNA extraction process. The extracted DNA is then transferred by EOP into the DNA amplification chamber where polymerase chain reaction (PCR) reagents, encapsulated in agarose, have been pre-loaded. Integration of DNA extraction and PCR amplification without the need for hydrodynamic pumping is achieved as well as long-term storage of the PCR reagents in the microfluidic device. © 2008 CBMS.