Deakin University
Browse

File(s) under permanent embargo

Epithelial cell folate is an accurate marker when compared with whole tissue biopsy folate for examining the role of folate status in colorectal cancer

conference contribution
posted on 2006-01-01, 00:00 authored by Sharleen O'ReillySharleen O'Reilly
Background – Epidemiological studies have shown low folate status is associated with colorectal cancer. Colonic tissue folate levels at different stages of cancer development should give important information, but different methodologies to extract the colonic tissue folates have been used. This has hampered progress in defining the relationship between systemic and tissue folate levels.
Objective – To evaluate two methods of colonic tissue preparation for estimation of total folate content.
Design – Whole tissue punch biopsy samples were obtained from the descending colon of 31individuals following a normal colonoscopy. Blood samples were obtained for the determination of plasma homocysteine (Hcy), red cell folate (RCF), methylenetetrahydrofolate reductase 677C>T genotype, and serum vitamin B12 and folate. Colonic tissue folate was measured both in washed whole tissue biopsies and in epithelial cells isolated from tissue biopsies.
Outcomes - Whole biopsy and epithelial cell folate concentrations were significantly correlated (R=.375; P=.038). Hcy was inversely correlated with both measures (R=-.365; P=.043 and R=-.364; P=.044 respectively). RCF was significantly correlated with isolated epithelial cell folate (R=.477; P=.007) but not with whole tissue biopsy folate (R=.264; P=.151). There were no significant associations between serum and colonic folate in this study.
Conclusion - Both methods are useful for comparing systemic and localised tissue folate status but epithelial cells may provide more reliable data.


History

Event

Nutrition Society of Australia. Scientific Meeting (30th :2006 : Sydney, N.S.W.)

Publisher

HEC Press

Location

Sydney, N.S.W.

Place of publication

Melbourne, Vic.

Start date

2006-11-29

End date

2006-12-02

ISSN

0964-7058

eISSN

1440-6047

Language

eng

Publication classification

E3.1 Extract of paper

Copyright notice

2001-2007, HEC PRESS

Title of proceedings

NSA 2006 : Nutrition through the Life Cycle : Proceedings of the Nutrition Society of Australia

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC