Fabrication of an integrated microfluidic device using multimaterial 3D printing for point-of-care detection of drugs in body fluids

© 17CBMS-0001. Multimaterial 3D printing was used to create an integrated microfluidic device containing two membranes with different porosity and electrodes in a single fabrication step capable of sample-in/answer-out analysis of small molecules in complex samples. A five-head fused deposition modeling (FDM) 3D printer was used to print the device in transparent acrylonitrile butadiene styrene (ABS), incorporating two different membrane materials (Lay-felt, Lay-gel), and a conductive material (carbon ABS). It was used to electrokinetically trap and concentrate target analytes in a microchannel where an electrophoretic separation was performed. The device aims for the analysis of small molecule drugs, and metabolites from biofluids.