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Integrated DNA purification and amplification using FTA® paper and PCR reagent encapsulation

conference contribution
posted on 2023-02-03, 04:29 authored by K J Shaw, R Vasiliadou, J Parton, N Pamme, Stephen Haswell
This paper reports the development of a combined microfluidic system for purification and amplification of DNA from FTA® paper. Using FTA® paper eradicates the need to elute nucleic acids from a solid-phase matrix prior to downstream processing, enabling both purification and amplification to be easily performed in a single chamber on the microfluidic device. Following optimization of the methodology and associated control systems, successful amplification of the D21 S11 locus from human buccal swab samples stored on FTA® paper was achieved. Such a system offers advantages in terms of a simple sample introduction interface and the ability to process archived samples in an integrated microfluidic environment with minimal risk of contamination.

History

Pagination

1396 - 1398

ISBN-13

9780979806452

Title of proceedings

Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012

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