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Investigation of plasmodiophora brassicae (clubroot disease) in vegetable brassica using arabidopsis thaliana as a model system

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conference contribution
posted on 2004-01-01, 00:00 authored by Arati Agarwal, V Kaul, Robert FaggianRobert Faggian, David CahillDavid Cahill
Clubroot, caused by Plasmodiophora brassicae, is the most devastating soil-borne disease of vegetable brassicas. It occurs all over the world and is responsible for crop losses of up to 10% every year. In Australia, the disease is being managed effectively with chemicals and cultural practices, but ideally control can be improved in the long term by the introduction of resistant cultivars. The life cycle ofP. brassicae and mode of action of plant resistance has not been fully elucidated because of the technical difficulties of working with an obligate, soil-borne plant pathogen. However, Arabidopsis thaliana, which is a host ofP. brassicae, has great potential as a model system for studying the life cycle, the infection process and development of resistance. We have developed a sand-liquid-culture system for growing Arabidopsis that allows easy observation of all life stages and, most importantly, the primary plasmodial stages within the root hair. The method was first optimised for observations of the lifecycle of the pathogen in a susceptible Arabidopsis ecotype (Col-3) where all stages of the lifecycle have now been observed and characterised. Further screening of Arabidopsis ecotypes for disease resistance has utilised one of the most virulent Australian pathotypes of brassica (ECD number 16/19/31). To date, Arabidopsis ecotype Ta-0 has shown a level of tolerance to the disease even though the roots get infected. It has been reported earlier that resistance toP. brassicae in Arabidopsis is due to one or a small number of genes. To examine changes in gene expression during the early, critical stages of infection, RNA was extracted from the susceptible and resistant ecotypes at two time points, 4 days and 17 days after inoculation. Microarray analysis will be used to investigate genome wide changes in gene expression during infection but also to identify candidate genes that may confer resistance to Australian isolates of the pathogen.

History

Event

ComBio2004 Conference (2004 : Perth, W.A.)

Pagination

142 - 142

Publisher

Australian Society for Biochemistry and Molecular Biology

Location

Perth, W.A.

Place of publication

[Perth, W.A.]

Start date

2004-09-29

End date

2004-09-30

ISSN

1328-4924

Language

eng

Notes

Reproduced with the kind permission of the copyright owner.

Publication classification

E3 Extract of paper

Copyright notice

2004, Australian Socity for Biochemistry and Molecular Biology

Title of proceedings

ComBio2004 : Abstracts of Papers Presented at the ComBio2004 Conference

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