Manufacturable system for zoonotic disease detection

Copyright © (2018) by Chemical and Biological Microsystems Society. All rights reserved. A manufacturable microfluidic system containing an injection-molded device for sample in/answer out analysis of a buccal swab lysate for avian influenza detection is presented. Fluidic processing was done using an external magnet to move paramagnetic particles, and the optimized injection moulding process allowed for side illuminated fluorescence detection with minimal hardware. The optimised amplification chemistry allowed for one-pot, truly isothermal Nucleic Acid Sequence Based Amplification. The chip design allows for simultaneous extraction, amplification and analysis of 5 targets. The system allows for the detection of 5000 copies of viral RNA (∼1.106copies/mL) using on-chip extraction of a 30 μL sample; 1.104 copies/mL with off-chip extraction.