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Molecular characterization and enzymatic hydrolysis of flavanoid extracted from citrus waste

conference contribution
posted on 2010-01-01, 00:00 authored by Munish PuriMunish Puri
The citrus fruit processing industry generates substantial quantities of waste rich in phenolic substances, which is a valuable natural source of polyphenols (flavonoids) such as naringin and its disposal is becoming a major problem. In the US alone, the juice processing of oranges and grapefruit generates over 5 Mt of citrus waste every year. In the case of India, about 2.15 Mt of citrus peel out of 6.28 Mt of citrus fruits are produced yearly from citrus juice processing. In case of Australia, about 15-40% of citrus peel waste is generated by processing of citrus fruit (0.85 Mt). Thus Isolation of functional compounds (mostly flavanoids) and their further processing can be of interest to the food and pharmaceutical industry. This peel is rich in naringin and may be used for rhamnose production by utilizing α-L-rhamnosidase (EC, an enzyme that catalyzes the cleavage of terminal rhamnosyl groups from naringin to yield prunin and rhamnose. We recently purified recombinant α-L-rhamnosidase from E. coli cells using immobilized metal-chelate affinity chromatography (IMAC) and used it for naringin hydrolysis. The purified enzyme established hydrolysis of naringin extracted from citrus peel and thus endorses its industrial applicability for producing rhamnose. Infrared (IR) spectroscopy confirmed molecular characteristics of naringin extracted from citrus peel waste.



International Symposium of Industrial Microbiology and Biotechnology (7th : 2010 : Melbourne, Victoria)


34 - 34


The Conference


Melbourne, Victoria

Place of publication

Melbourne, Victoria

Start date


End date





Abstract book website : 7th International Symposium on Industrial Micrbiology Biotechnology Program 26 June 2010.pdf

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E3 Extract of paper


M Britz

Title of proceedings

Abstract booklet of the 7th International Symposium of Industrial Microbiology and Biotechnology

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