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Nanofluidic trap for DNA extraction from biological samples
conference contribution
posted on 2015-01-01, 00:00 authored by A I Shallan, Rosanne GuijtRosanne Guijt, M C BreadmoreA new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.
History
Event
Miniaturized systems for chemistry and life sciences. International conference (19th : 2015 : Seoul, Korea)Pagination
1963 - 1965Publisher
[Chemical and Biological Microsystems Society]Location
Seoul, KoreaPlace of publication
[Seoul, Korea]Start date
2015-10-25End date
2015-10-29eISSN
1556-5904ISBN-13
9780979806483Language
engPublication classification
E Conference publication; E1.1 Full written paper - refereedCopyright notice
2015 by the Chemical and Biological Microsystems SocietyEditor/Contributor(s)
J-K Park, M Tokeshi, Y-K Cho, D-P Kim, Q Fang, Y NamTitle of proceedings
MicroTAS 2015 - Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life SciencesUsage metrics
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