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Nanofluidic trap for DNA extraction from biological samples

Version 2 2024-06-04, 12:16
Version 1 2018-10-23, 16:16
conference contribution
posted on 2024-06-04, 12:16 authored by AI Shallan, Rosanne GuijtRosanne Guijt, MC Breadmore
A new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.

History

Pagination

1963-1965

Location

Seoul, Korea

Start date

2015-10-25

End date

2015-10-29

eISSN

1556-5904

ISBN-13

9780979806483

Language

eng

Publication classification

E Conference publication, E1.1 Full written paper - refereed

Copyright notice

2015 by the Chemical and Biological Microsystems Society

Editor/Contributor(s)

Park J-K, Tokeshi M, Cho Y-K, Kim D-P, Fang Q, Nam Y

Title of proceedings

MicroTAS 2015 - Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Event

Miniaturized systems for chemistry and life sciences. International conference (19th : 2015 : Seoul, Korea)

Publisher

[Chemical and Biological Microsystems Society]

Place of publication

[Seoul, Korea]

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