A new device seamlessly integrating various electrokinetic processes enables the analysis of short ssDNA (20 bases) directly from whole blood. An electrokinetic trap was integrated to achieve extraction, concentration, and desalting of ssDNA in 200 s followed by a 90 s electrophoretic separation. The trap consists of two sequentially coupled nanojunctions with different pore size and exhibits selective ion transport properties. Depletion forces near the nanojunctions and the apparent electromigration were tuned to enable the trapping and subsequent analysis of fluorescein labeled-ssDNA. The quantitation limit (S/N ratio = 10) was 12.5 nM using 40 μL of spiked whole blood.
History
Pagination
1963-1965
Location
Seoul, Korea
Start date
2015-10-25
End date
2015-10-29
eISSN
1556-5904
ISBN-13
9780979806483
Language
eng
Publication classification
E Conference publication, E1.1 Full written paper - refereed
Copyright notice
2015 by the Chemical and Biological Microsystems Society
Editor/Contributor(s)
Park J-K, Tokeshi M, Cho Y-K, Kim D-P, Fang Q, Nam Y
Title of proceedings
MicroTAS 2015 - Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Event
Miniaturized systems for chemistry and life sciences. International conference (19th : 2015 : Seoul, Korea)