File(s) under permanent embargo
Towards a colony counting system using hyperspectral imaging
conference contribution
posted on 2012-02-13, 00:00 authored by B Masschelein, Antonio Robles-KellyAntonio Robles-Kelly, C Blanch, N Tack, B Simpson-Young, A LambrechtsColony counting is a procedure used in microbiology laboratories for food quality monitoring, environmental management, etc. Its purpose is to detect the level of contamination due to the presence and growth of bacteria, yeasts and molds in a given product. Current automated counters require a tedious training and setup procedure per product and bacteria type and do not cope well with diversity. This contrasts with the setting at microbiology laboratories, where a wide variety of food and bacteria types have to be screened on a daily basis. To overcome the limitations of current systems, we propose the use of hyperspectral imaging technology and examine the spectral variations induced by factors such as illumination, bacteria type, food source and age and type of the agar. To this end, we perform experiments making use of two alternative hyperspectral processing pipelines and compare our classification results to those yielded by color imagery. Our results show that colony counting may be automated through the automatic recovery of the illuminant power spectrum and reflectance. This is consistent with the notion that the recovery of the illuminant should minimize the variations in the spectra due to reflections, shadows and other photometric artifacts. We also illustrate how, with the reflectance at hand, the colonies can be counted making use of classical segmentation and classification algorithms.
History
Event
SPIE BiOS - Imaging, Manipulation and Analysis of Biomolecules, Cells and Tissues X. International Conference (2012. San Francisco, California)Volume
8225Publisher
S P I E - International Society for Optical EngineeringLocation
San Francisco, CaliforniaPlace of publication
Bellingham, Wash.Publisher DOI
Start date
2012-01-21End date
2012-01-26ISSN
0277-786XLanguage
engPublication classification
E1.1 Full written paper - refereedCopyright notice
2012, SPIEEditor/Contributor(s)
Daniel Farkas, Dan Nicolau, Robert LeifTitle of proceedings
SPIE BiOS 2012 : Proceedings of SPIE BiOS Conference on Imaging, Manipulation and Analysis of Biomolecules, Cells, and Tissues XUsage metrics
Categories
No categories selectedKeywords
Licence
Exports
RefWorks
BibTeX
Ref. manager
Endnote
DataCite
NLM
DC