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Wall-less stationary ph boundary for stacking proteins on a glass microchip
conference contribution
posted on 2015-01-01, 00:00 authored by H H See, Rosanne GuijtRosanne Guijt, M C BreadmoreThis work reports a new electrophoretic stacking protocol based on the formation of a stationary pH boundary to concentrate and analyze proteins on a glass microchip. The feasibility of continuously loading proteins from a continuously flowing sample to increase the amount of analyte injected prior to separation and detection was demonstrated. An increase in protein concentration of about 800-1000 fold was achieved in approximately 5 min of stacking, and the subsequent separation and detection of three different protein bands namely BSA, β-lactoglobulin and as r-phycoerthrin were achieved within 3 min.
History
Event
Miniaturized systems for chemistry and life sciences. International conference (19th : 2015 : Seoul, Korea)Pagination
1969 - 1971Publisher
[Chemical and Biological Microsystems Society]Location
Seoul, KoreaPlace of publication
[Seoul, Korea]Start date
2015-10-25End date
2019-10-29eISSN
1556-5904ISBN-13
9780979806483Language
engPublication classification
E Conference publication; E1.1 Full written paper - refereedCopyright notice
2015 by the Chemical and Biological Microsystems SocietyEditor/Contributor(s)
J-K Park, M Tokeshi, Y-K Cho, D-P Kim, Q Fang, Y NamTitle of proceedings
MicroTAS 2015 - Proceedings of the 19th International Conference on Miniaturized Systems for Chemistry and Life SciencesUsage metrics
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