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1000 spider silkomes: Linking sequences to silk physical properties

journal contribution
posted on 2024-02-08, 04:06 authored by K Arakawa, N Kono, AD Malay, A Tateishi, N Ifuku, H Masunaga, R Sato, K Tsuchiya, R Ohtoshi, D Pedrazzoli, A Shinohara, Y Ito, H Nakamura, A Tanikawa, Y Suzuki, T Ichikawa, S Fujita, M Fujiwara, M Tomita, SJ Blamires, JA Chuah, H Craig, CP Foong, G Greco, J Guan, C Holland, DL Kaplan, K Sudesh, BB Mandal, Y Norma-Rashid, NA Oktaviani, RC Preda, NM Pugno, R Rajkhowa, X Wang, K Yazawa, Z Zheng, K Numata
Spider silks are among the toughest known materials and thus provide models for renewable, biodegradable, and sustainable biopolymers. However, the entirety of their diversity still remains elusive, and silks that exceed the performance limits of industrial fibers are constantly being found. We obtained transcriptome assemblies from 1098 species of spiders to comprehensively catalog silk gene sequences and measured the mechanical, thermal, structural, and hydration properties of the dragline silks of 446 species. The combination of these silk protein genotype-phenotype data revealed essential contributions of multicomponent structures with major ampullate spidroin 1 to 3 paralogs in high-performance dragline silks and numerous amino acid motifs contributing to each of the measured properties. We hope that our global sampling, comprehensive testing, integrated analysis, and open data will provide a solid starting point for future biomaterial designs.

History

Journal

Science Advances

Volume

8

Article number

ARTN eabo6043

Location

United States

ISSN

2375-2548

eISSN

2375-2548

Language

English

Publication classification

C1 Refereed article in a scholarly journal

Issue

41

Publisher

AMER ASSOC ADVANCEMENT SCIENCE

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