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3T3-L1 adipocytes display phenotypic characteristics of multiple adipocyte lineages

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journal contribution
posted on 2015-01-01, 00:00 authored by Shona MorrisonShona Morrison, Sean McgeeSean Mcgee
Differentiated 3T3-L1 adipocytes are a widely used in vitro model of white adipocytes. In addition to classical white and brown adipocytes that are derived from different cell lineages, beige adipocytes have also been identified, which have characteristics of both white and brown adipocytes. Here we show that 3T3-L1 adipocytes display features of multiple adipocytes lineages. While the gene expression profile and basal bioenergetics of 3T3-L1 adipocytes was typical of white adipocytes, they responded acutely to catecholamines by increasing oxygen consumption in an UCP1-dependent manner, and by increasing the expression of genes enriched in brown but not beige adipocytes. Chronic exposure to catecholamines exacerbated this phenotype. However, a beige adipocyte differentiation procedure did not induce a beige adipocyte phenotype in 3T3-L1 fibroblasts. These multiple lineage features should be considered when interpreting data from experiments utilizing 3T3-L1 adipocytes.

History

Journal

Adipocyte

Volume

4

Pagination

295-302

Location

London, Eng.

ISSN

2162-397X

Language

eng

Publication classification

C Journal article, C1 Refereed article in a scholarly journal

Copyright notice

2015, Taylor & Francis

Issue

4

Publisher

Taylor & Francis

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