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AMP and adenosine are both ligands for adenosine 2B receptor signaling
journal contribution
posted on 2018-01-15, 00:00 authored by Jessica K Holien, Benjamin Seibt, Veena Roberts, Evelyn Salvaris, Michael W Parker, Peter J Cowan, Karen DwyerKaren DwyerAdenosine is considered the canonical ligand for the adenosine 2B receptor (A2BR). A2BR is upregulated following kidney ischemia augmenting post ischemic blood flow and limiting tubular injury. In this context the beneficial effect of A2BR signaling has been attributed to an increase in the pericellular concentration of adenosine. However, following renal ischemia both kidney adenosine monophosphate (AMP) and adenosine levels are substantially increased. Using computational modeling and calcium mobilization assays, we investigated whether AMP could also be a ligand for A2BR. The computational modeling suggested that AMP interacts with more favorable energy to A2BR compared with adenosine. Furthermore, AMPαS, a non-hydrolyzable form of AMP, increased calcium uptake by Chinese hamster ovary (CHO) cells expressing the human A2BR, indicating preferential signaling via the Gq pathway. Therefore, a putative AMP-A2BR interaction is supported by the computational modeling data and the biological results suggest this interaction involves preferential Gq activation. These data provide further insights into the role of purinergic signaling in the pathophysiology of renal IRI.
History
Journal
Bioorganic and medicinal chemistry lettersVolume
28Issue
2Pagination
202 - 206Publisher
ElsevierLocation
Amsterdam, The NetherlandsPublisher DOI
ISSN
0960-894XeISSN
1464-3405Language
engPublication classification
C Journal article; C1.1 Refereed article in a scholarly journalCopyright notice
2017, ElsevierUsage metrics
Keywords
A(2B)RAMPAdenosineDockingG(q) activationHomology modelAdenosine MonophosphateAnimalsCHO CellsCricetulusDose-Response Relationship, DrugHumansLigandsMolecular Docking SimulationMolecular StructureReceptor, Adenosine A2BSignal TransductionStructure-Activity RelationshipScience & TechnologyLife Sciences & BiomedicinePhysical SciencesChemistry, MedicinalChemistry, OrganicPharmacology & PharmacyChemistryA2B RECEPTORAGONISTACTIVATIONEXPRESSIONPROTECTSKIDNEYINJURYOrganic Chemistry
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