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AMP and adenosine are both ligands for adenosine 2B receptor signaling

Version 2 2024-06-03, 18:12
Version 1 2018-07-11, 15:39
journal contribution
posted on 2024-06-03, 18:12 authored by Jessica K Holien, Benjamin Seibt, Veena Roberts, Evelyn Salvaris, Michael W Parker, Peter J Cowan, Karen M Dwyer
Adenosine is considered the canonical ligand for the adenosine 2B receptor (A2BR). A2BR is upregulated following kidney ischemia augmenting post ischemic blood flow and limiting tubular injury. In this context the beneficial effect of A2BR signaling has been attributed to an increase in the pericellular concentration of adenosine. However, following renal ischemia both kidney adenosine monophosphate (AMP) and adenosine levels are substantially increased. Using computational modeling and calcium mobilization assays, we investigated whether AMP could also be a ligand for A2BR. The computational modeling suggested that AMP interacts with more favorable energy to A2BR compared with adenosine. Furthermore, AMPαS, a non-hydrolyzable form of AMP, increased calcium uptake by Chinese hamster ovary (CHO) cells expressing the human A2BR, indicating preferential signaling via the Gq pathway. Therefore, a putative AMP-A2BR interaction is supported by the computational modeling data and the biological results suggest this interaction involves preferential Gq activation. These data provide further insights into the role of purinergic signaling in the pathophysiology of renal IRI.

History

Journal

Bioorganic and medicinal chemistry letters

Volume

28

Pagination

202-206

Location

Amsterdam, The Netherlands

ISSN

0960-894X

eISSN

1464-3405

Language

eng

Publication classification

C Journal article, C1.1 Refereed article in a scholarly journal

Copyright notice

2017, Elsevier

Issue

2

Publisher

Elsevier