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A complete set of repeat-depleted, PCR-amplifiable, human chromosome-specific painting probes

journal contribution
posted on 1999-01-01, 00:00 authored by A Bolzer, Jeffrey CraigJeffrey Craig, T Cremer, M R Speicher
We describe the generation of a complete set of human chromosome-specific painting probes depleted in repetitive sequences. These probes yield highly specific signals when hybridized without the addition of a blocking agent, such as Cot-1 DNA, and without probe preannealing prior to hybridization. Fluorescent intensities and signal-to-background ratios for these probes are comparable to those of untreated probes hybridized with Cot-1 DNA. We demonstrate the suitability of these probes for applications with very complex probe sets, such as multiplex-FISH.

History

Journal

Cytogenetic and genome research

Volume

84

Issue

3-4

Pagination

233 - 240

Publisher

Karger

Location

Basel, Switzerland

ISSN

0301-0171

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

1997, S. Karger AG, Basel