Deakin University
Browse

File(s) under permanent embargo

A discussion on the process of defining 2-D separation selectivity

journal contribution
posted on 2010-06-01, 00:00 authored by Paul Stevenson, M Mnatsakanyan, A Francis, R Shalliker
In this manuscript, we investigate the importance that must be placed on the selection of standard compounds when undertaking studies to optimize the performance of 2-D-HPLC separations. A geometric approach to factor analysis and a measure of peak density across the separation space were applied to assess localized measures of component distributions within the 2-D separation plane. The results of this analysis of data showed that the measure of separation quality varied markedly, depending on the elution zone for which the test was undertaken. The study concluded that if standards cannot be obtained that adequately describe the entire sample matrix, the sample itself should be used, and also, the separation should be optimized for regions of interest, not necessarily the separation as a whole.

History

Journal

Journal of separation science

Volume

33

Issue

10

Pagination

1405 - 1413

Publisher

Wiley - V C H Verlag GmbH & Co. KGaA

Location

Weinheim, Germany

ISSN

1615-9306

eISSN

1615-9314

Language

eng

Publication classification

C1.1 Refereed article in a scholarly journal

Copyright notice

2010, Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim

Usage metrics

    Research Publications

    Categories

    No categories selected

    Exports

    RefWorks
    BibTeX
    Ref. manager
    Endnote
    DataCite
    NLM
    DC