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A fourth Sp1 site in the human immunodeficiency virus type 1 long terminal repeat is essential for negative-sense transcription
journal contribution
posted on 1996-10-01, 00:00 authored by Anna PeetersAnna Peeters, P F Lambert, N J DeaconWe report the discovery of a fourth Sp1 binding site at the 5' end of the U3 region of the human immunodeficiency virus type 1 (HIV-1) long terminal repeat (HIV-1 Sp1 IV), localized to HXB2 nucleotides -433 to -441. This site is shown to bind Sp1 protein specifically in electrophoretic mobility shift assays. Sp1 protein appears to bind to HIV-1 Spl IV with 5 to 10 times lower affinity than to a consensus Sp1 site. Mutation of HIV-1 Sp1 IV in an HXB2-derived long terminal repeat-chloramphenicol acetyltransferase reporter construct gave no significant change in positive-sense transcription but abolished both basal and phorbol myristate acetate-activated negative-sense transcription. Taken together, the results further define the HIV-1 negative-sense promoter as an Sp1-dependent, phorbol myristate acetate-responsive, and Tat-inhibited promoter initiating at HXB2 nucleotide -450.
History
Journal
Journal of virologyVolume
70Issue
10Pagination
6665 - 6672Publisher
American Society for MicrobiologyLocation
Washington, D.C.ISSN
0022-538XeISSN
1098-5514Language
engPublication classification
C1.1 Refereed article in a scholarly journalCopyright notice
1996, American Society for MicrobiologyUsage metrics
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